Feng Zhang lab’s recent work published in Science introduces a novel algorithm, fast locality-sensitive hashing-based clustering (FLSHclust), designed for mining large sequencing databases. This approach aims to address the limitations of existing methods in discovering rare protein families due to the exponential growth of databases. The research focused on systematically enumerating CRISPR-linked gene modules across all available sequencing data. CRISPR-Cas systems, known for their role in microbial immune defense and as the basis for genome editing technologies, have recently been linked to diverse biochemical activities like transposition and protease activity. The study hypothesizes that many more enzymatic activities associated with CRISPR systems could be discovered, some of which might be rare in existing databases.
Using FLSHclust, the researchers identified 188 previously unknown CRISPR-associated systems, including several rare ones. They experimentally characterized four new systems: a type IV system with an HNH nuclease domain, demonstrating a specific interference mechanism; two type I systems with HNH domains for precise DNA cleavage, which showed potential for genome editing in human cells; and candidate type VII systems indicating RNA targeting origins from type III-E CRISPR systems. Additionally, they discovered new effector and adaptation components, associations of Mu transposons with CRISPR systems, and proteins related to type V systems. They also noted a potential anti-CRISPR mechanism involving Cas9 and other non-CRISPR repeat arrays.
The study underscores the potential of FLSHclust in efficiently clustering millions of sequences and highlights the discovery of diverse biochemical activities linked to RNA-guided mechanisms in CRISPR systems, opening avenues for biotechnological advancements.
PackGene Biotech is a world-leading CRO and CDMO, excelling in AAV vectors, mRNA, plasmid DNA, and lentiviral vector solutions. Our comprehensive offerings span from vector design and construction to AAV, lentivirus, and mRNA services. With a sharp focus on early-stage drug discovery, preclinical development, and cell and gene therapy trials, we deliver cost-effective, dependable, and scalable production solutions. Leveraging our groundbreaking π-alpha 293 AAV high-yield platform, we amplify AAV production by up to 10-fold, yielding up to 1e+17vg per batch to meet diverse commercial and clinical project needs. Moreover, our tailored mRNA and LNP products and services cater to every stage of drug and vaccine development, from research to GMP production, providing a seamless, end-to-end solution.
Related News
NeuExcell Therapeutics Unveils Breakthrough in Stroke Treatment at ASGCT 2024
In a groundbreaking development, NeuExcell Therapeutics has revealed a significant breakthrough in its NXL-001 product for stroke treatment. The announcement was made at the 2024 American Society of Gene and Cell Therapy (ASGCT) Annual Meeting, where the company...
Unraveling CRISPR Precision: BreakTag Illuminates Pathways to Improved Gene Editing
Introduction:The quest for precision in CRISPR-Cas9 gene editing takes a significant leap forward with the development of BreakTag, a method devised to enhance our understanding of DNA double-strand breaks (DSBs) induced by Cas9. This breakthrough, detailed in a...
Sumitomo Pharma Announces FDA Acceptance of Supplemental New Drug Application for Vibegron in Men with Overactive Bladder Symptoms Receiving Pharmacological Therapy for Benign Prostatic Hyperplasia
–Supplemental New Drug Application (sNDA) submission based on Phase 3 study of vibegron 75mg (GEMTESA) demonstrating statistically significant reductions in daily micturition and urgency episodes– –If approved, vibegron will be the first and only beta-3 agonist for...
Herpes cure with gene editing makes progress in laboratory studies
Herpes simplex virus. Credit: CDCResearchers at Fred Hutch Cancer Center have found in pre-clinical studies that an experimental gene therapy for genital and oral herpes removed 90% or more of the infection and suppressed how much virus can be released from an...
Related Services