Research Grade Plasmid Preparation

Overview

Plasmid end products prepared through PackGene’s Plasmid Extraction service are viable for transfection application across the discovery and preclinical research phases.

The quality of plasmid will have a direct impact on the experimental outcome of downstream applications. With years of experience and process optimization PackGene has developed a robust protocol for plasmid preparation services accommodating to both small research labs to biotech and pharmaceutical companies. Choose from our high-quality plasmid from 100ug to 1000mg and up, with high homogeneity and ultra-low endotoxin level (<10EU/mg).

Highlights
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Bulk extraction volumes from 10ug to 1000mg

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Endotoxin less than 100 EU/mg

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Broad applicability, including cellular assays, viral dissemination, CAR-T and other vector production projects

Services
Plasmid Grade
Scale Timeline (business days)
100ug 3~5
200ug 3~5
500ug 3~5
1mg 5~7
2mg 5~7
10mg 8~10
20mg 8~10
50mg 8~10
AAV
Plasmid QC
plasmid QC
Standard Analytical Method
Homogeneity Predominantly Supercoiled Densitometry upon agarose gel electrophoresis
Appearance Clear, no visible particles Visual Inspection
A260/280 1.80~2.00 Nanodrop UV absorption
Restriction Analysis Conforms to reference Enzyme digestion and electrophoresis
Endotoxin <100EU/mg or <10EU/mg Spot check

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