Unlock the potential of plasmid DNA across multiple therapeutic approaches, from gene and cell therapies, viral vector production, and immunotherapeutics to mRNA/DNA vaccines and nucleic acid drugs. PackGene provides plasmid preparation services that are ideal for any phase of the drug development cycle including basic discovery research through commercialization at any scale.
Flexible Sizing
We offer production sizes from 100µg to 2g for preclinical grades of plasmid and up to 100g for GMP plasmid production.
Precision
Speed
Wide Compatibility
We offer plasmid production grades that are ideal for all drug development stages, from discovery research to commercialization at any scale. Choose from two plasmid grades for discovery and preclinical work. For clinical stage work, please see GMP plasmid manufacturing
Tailored QC Tests
Our range of quality control tests for endotoxin levels, supercoil content, residual contaminants, and sequence fidelity can be customized for your workflow.
Support for Various Projects
Our plasmids help enable cell transfection, protein expression, AAV packaging, mRNA production, viral vector production, vaccine development, antibody production, and other preclinical processes.
| Research Grade | Preclinical Grade | |
| Application | Molecular cloning, protein expression, transfection, viral packaging for research | Large scale viral packaging, animal studies |
| Scale | 100µg-100mg | 10mg-2g |
| Timeline | as fast as 3 days | as fast as 2-3 weeks |
| QC standards | ||
| Appearance | Clear, colorless, no visible particulates | |
| A260:280 | 1.8 – 2.0 | |
| Homogeneity | Predominantly supercoiled | >90% HPLC |
| Restriction Analysis | Conforming to reference pattern | |
| Residual RNA | N/A | Undetected by SYBRGold |
| Residual E. coli DNA | N/A | Quantitative PCR ≤1% |
| Endotoxin | ≤100 EU/mg, optional LAL test* | ≤10 EU/mg |
| Bioburden Testing | Optional* | No growth on agarose plate after 48 hours |
| Sequencing | Optional* | GOI |
| Antibiotics Free Production | No | Optional |
| Residual Host Protein | – | <0.1% by HCP ELISA* |
| Animal Free Production* | – | TSE/BSE* |
| Mycoplasma Contamination | – | Quantitative PCR, Negative * |
| Material Archiving | Yes (plasmid only) | Yes (plasmid + bacteria) upon request * |
| pH | – | Potentiometry 7.5~8.0, USP <791> * |
| Kan | – | ELISA, <0.5ng/g * |
| Sterility | – | USP <71>* |
| Osmolality | – | USP <785> * |
| Advanced Endotoxin Removal <0.005 EU/µg | – | Available with additional charge |
| Dedicated purification resin | – | Upon request* |
| Buffer | ddH2O, TE or customer defined | |
| Dispensing | Upon request | |
| Dedicated filtration membrane | – | Sterile filtration* |
*Tests marked need additional charges and time.
Synthesize new plasmids or re-prep previously synthesized ones.
Research Grade Plasmid
Fast, affordable, low-endotoxin plasmid DNA for viral vector engineering, high-throughput screening, or mRNA production among other applications.
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Preclinical Grade Plasmid
Rigorous quality standards and analysis for your preclinical endeavors. Tailored for preclinical in vivo animal studies and suitable for a range of species from mice to non-human primates. Available in quantities starting from 10 mg.
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