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AAV-shRNA Cloning

AAV-shRNA Interference

PackGene provides AAV vectors for shRNA (short-hairpin RNA) packaging, one of the most widely used methods in RNAi experiments. By transcribing small interfering RNA fragments via H1, U6 promoters and choosing various protein expression tags, we can inspect the AAV in vivo transfection.

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RNA interference (RNAi), a highly specific tool for gene deletion research, is a double-stranded RNA (dsRNA)-mediated gene silencing phenomenon discovered by Andrew Fire and Craig Mello C. Elegans in nematodes. It is present in most eukaryotes and has been widely used for gene function studies, drug discovery and gene silencing therapy in various eukaryotes.

In recent years, several trials have shown that RNAi drugs targeting disease-causing genes have great potential for the treatment of human diseases. In contrast to other methods, the use of AAV vectors to express shRNA to cause down-regulation of gene expression can regulate target genes in live animals for a long time, which is a common method for genetic studies in live animals. h1, U6 promoters are commonly used to transcribe small interfering RNA fragments. Researchers believe that the H1 promoter is less toxic and could lead to more reliable results in RNAi interference experiments.

PackGene provides library numbers for shRNA target genes and required vectors. In addition, we offer target design, mapping and vector construction. If the target has not been validated, we recommend selecting 2-3 more candidate targets.

PackGene’s guaranteed quality with our free extra two designs:

  • Under the premise that the transfection efficiency of the target cells reaches 90 per cent (70%-90%), for coding genes, one mRNA level of 70% or more is guaranteed in the three interfering sequences, protein level is not guaranteed; for non-coding genes, no commitment to interfere with the efficiency;
  • Under this condition, if the three interfering sequences are not effective at the mRNA level, PackGene will design two sequences for you free of charge after you provides the original data.