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AAV-shRNA Cloning

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AAV-shRNA Interference

PackGene provides AAV vectors for reducing protein translation via short-hairpin RNA (shRNA), one of the most widely used RNAi methods inhibiting protein translation. We offer options to express shRNA under the H1 or U6 promoter, and all of our AAV-shRNA vectors are designed to co-express a reporter protein of your choosing.

Interested in our AAV services? Please contact us for a free consultation or  submit a request for a quotation.

RNA interference (RNAi) is a highly specific tool for protein knockdown in which via small non-coding RNA bind to target proteins mRNA to drive mRNA degradation and therefore reduce protein translation. RNAi mechanisms are endogenously present in most eukaryotes, and have been widely adopted as a molecular tool for gene function studies, drug discovery, and gene silencing therapy.

Several recent trials have shown that RNAi therapies that target disease-causing genes hold great potential for the treatment of human diseases. In comparison to other delivery methods, AAV vector-based expression of RNAi has been shown to drive prolonged reduction of mRNA targets in live animals.

PackGene can assist customers with shRNA vector design and vector construction. Please inquire for more details (info@packgene.com).

PackGene’s AAV-shRNA Guarantee:

  • PackGene offers a guarantee that our tripleshRNA expression AAVs will reduce target mRNA expression by 70% or more in cases where target-cell transfection efficiency is 90% or greater*.
  • In a case where our AAV-shRNAguarantee is not met, PackGene will design two new interfering sequences for you free of charge**.

* Protein expression level is not guaranteed. Disruptions in the efficacy of non-coding genes is not guaranteed.
** Customer must provide clear data demonstrating inefficiency of mRNA knockdown.