Severe acute respiratory syndrome (SARS) is a debilitating and dangerous human syndrome caused by coronavirus infection. Coronaviruses enters the human body by binding to angiotensin-converting enzyme 2 (ACE2), and thus animal models that express ACE2 are useful for the study of coronavirus associated respiratory diseases. Traditional knock-in animal models are labor-intensive and require at least six months to build as well as extensive resource allocations to maintain. PackGene has generated AAV-based animal models that overexpress the hACE2 protein in specific organs, making them suitable for coronavirus studies including COVID-19-related studies.
PackGene’s model takes advantage of rAAV serotype tropisms to selectively infect specific tissues in mice for generating AAV-hACE2 animal models. AAV infected tissues show continuous and stable expression of the hACE2 protein producing in a simple and timesaving method for new coronavirus-related drug development and research.
Comparison between AAV-hACE2 animal modeling and traditional animal modeling:
| AAV-hACE2 animal modeling |
Traditional animal modeling |
|
| Production Cycle | Short cycle within 14 days | Over 6 months |
| Process | Simple | Complicated steps |
| Histotropism | Specific distribution | Wide distribution |
Injection Method: Intranasal Administration
Recommended Dose: 2×10^11GC/ml, 40μl
Animal Model: 6-8-week-old female BALB/c mice
Journal: Antiviral Research, 2022 (IF=10.103)
Paper Title: Rational design of AAVrh10-vectored ACE2 functional domain to broadly block the cell entry of SARS-CoV-2 variants
DOI: https://doi.org/10.1016/j.antiviral.2022.105383
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