AAV-CRISPR

Overview

CRISPR is a state-of-the-art genetic modification tool that can be used to knockout, mutate, or overexpress a gene of interest. CRISPR based research strategies have been widely employed across biological fields of study due to their powerful performance and relatively straightforward mechanism of action.

CRISPR gene editing requires the exogenous expression of the Cas9 enzyme as well as a 15-20nt guide RNA that is specifically targeted to the experiment’s gene of interest. Recent advances have made the design, construction, and use of the CRISPR systems increasingly convenient. PackGene’s pre-made Cas9 vectors build on this convenience by providing packaged AAV vectors for Cas9 expression at lighting fast turnaround times.

PackGene provides pre-made “off the shelf” CRISPR-AAV vectors that drive Cas9 expression under the EFS, miniCMV, or CMV broad-spectrum promoters as well as tissue-specific promoters in the AAV9 serotype. Our in stock pre-made products are shipped within one business day, making these products ideal for projects that are on a short timeline and require such standardized vectors.

PackGene provides high quality packaged AAVs for CRISPR Cas9 or CRISPR Cpf1 expression that can be directly applied in vivo and include a complete quality inspection report.

Products

SKU

Serotype

Vector

Titer & volume

AAV9-A2

AAV9

EFS.SpCas9

≥1E+13 GC/mL, 0.1 mL

AAV9-B2

EFS.SpCas9HF

AAV9-B202

miniCMV.SpCas9HF

AAV9-C202

miniCMV.SaCas9

AAV9-C2

CMV.SaCas9

AAV9-D2

CMV.SaCas9HF

AAV9-G101

EFS.AsCpf1

AAV9-H101

EFS.LbCpf1

AAV9-G102

miniCMV.AsCpf1

AAV9-H102

miniCMV.LbCpf1

AAV9-C202*

EFS.SaCas9

AAV9-C206

AAV9

Elastase I.SaCas9

≥1E+13 GC/mL, 0.1 mL

AAV9-C207

rInsulin 2.SaCas9

AAV9-C208

mInsulin2.SaCas9

AAV9-C209

hDesmin.SaCas9

AAV9-C210

hSynapsin.SaCas9

AAV9-C212

MECP2.SaCas9

AAV9-D2*

CMV.SaCas9HF

AAV9-D202

EFS.SaCas9HF

AAV9-D206

Elastase I.SaCas9HF

AAV9-D207

rInsulin 2.SaCas9HF

AAV9-D208

mInsulin2.SaCas9

AAV9-D209

hDesmin.SaCas9
AAV9-D210 hSynapsin.SaCas9
AAV9-D212 MECP2.SaCas9

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AAV

PackGene’s off the shelf Cas9 and Cpf1 vectors are packaged in AAV9, and can be directly, conveniently, and efficiently used for in vivo experiments. Each off the shelf product is delivered with a complete quality inspection report.

Case Study
Products: CRISPR AAV vectors from PackGene Biotech
Injection Method: Intramuscular Injection
Recommended Dose: 25 μL, 1.5E+12 vg/ml
Targeting Site: muscle
Animal Model: DMD mouse model
Journal: Genome Medicine, 2021 (IF=15.266)
Paper Title: In vivo genome editing in mouse restores dystrophin expression in Duchenne muscular dystrophy patient muscle fibers
DOI: 10.1186/s13073-021-00876-0
AAV production and injection
CRISPR AAV vectors were generated by PackGene Biotech Co. (Guangzhou, China). For intramuscular injection into NSI mice, the animals were anesthetized by abdominal injection of 5% chloral hydrate, followed by
injection of CRISPR AAV vector (25 μL, 1.5E+12 vg) into the TA muscle transplanted with DMD–MDSCs.
More Study Cases of PackGene’s AAV Services, please click here

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