AAV-CRISPR

PackGene provides pre-made CRISPR-AAV vectors based on AAV9 serotypes, and cover EFS/miniCMV/CMV broad-spectrum promoters and tissue-specific promoters. Without waiting, our products in stock can be shipped within the same working day or the nearest working day, making it an ideal choice for gene editing experiments in emergency.
CRISPR gene editing is a breakthrough after TALEN and ZFN technologies. It uses a gRNA to guide nucleases (such as Cas9) for specific editing of target genes. CRISPR-Cas9 system shows advantages like high editing efficiency, operator-friendly and rich designability. The construction and use of gRNA or Cas9 vectors are now very convenient, and they have been used in gene editing experiments in mammals, plants and other species.
PackGene provides packaged AAV based on CRISPR Cas9 or CRISPR Cpf1 , which can be directly applied to animal experiments. And we provides a complete quality inspection report.
SKU |
Serotype |
Vector |
Titer and volume |
AAV9-A2 |
AAV9 |
EFS.SpCas9 |
≥1E+13 GC/mL, 0.1 mL |
AAV9-B2 |
EFS.SpCas9HF |
||
AAV9-B202 |
miniCMV.SpCas9HF |
||
AAV9-C202 |
miniCMV.SaCas9 |
||
AAV9-C2 |
CMV.SaCas9 |
||
AAV9-D2 |
CMV.SaCas9HF |
||
AAV9-G101 |
EFS.AsCpf1 |
||
AAV9-H101 |
EFS.LbCpf1 |
||
AAV9-G102 |
miniCMV.AsCpf1 |
||
AAV9-H102 |
miniCMV.LbCpf1 |
||
AAV9-C202* |
EFS.SaCas9 |
||
AAV9-C206 |
AAV9 |
Elastase I.SaCas9 |
≥1E+13 GC/mL, 0.1 mL |
AAV9-C207 |
rInsulin 2.SaCas9 |
||
AAV9-C208 |
mInsulin2.SaCas9 |
||
AAV9-C209 |
hDesmin.SaCas9 |
||
AAV9-C210 |
hSynapsin.SaCas9 |
||
AAV9-C212 |
MECP2.SaCas9 |
||
AAV9-D2* |
CMV.SaCas9HF |
||
AAV9-D202 |
EFS.SaCas9HF |
||
AAV9-D206 |
Elastase I.SaCas9HF |
||
AAV9-D207 |
rInsulin 2.SaCas9HF |
||
AAV9-D208 |
mInsulin2.SaCas9 |
||
AAV9-D209 |
hDesmin.SaCas9 |
||
AAV9-D210 |
hSynapsin.SaCas9 |
||
AAV9-D212 |
MECP2.SaCas9 |
PackGene provides Cas9 and Cpf1 vectors packaged with AAV, which can be directly, conveniently and efficiently applied to animal experiments. Each spot fast service provides a complete quality inspection report.
Case Study
Injection Method: Intramuscular Injection
Recommended Dose: 25 μL, 1.5E+12 vg/ml
Targeting Site: muscle
Animal Model: DMD mouse model
Journal: Genome Medicine, 2021 (IF=15.266)
Paper Title: In vivo genome editing in mouse restores dystrophin expression in Duchenne muscular dystrophy patient muscle fibers
DOI: 10.1186/s13073-021-00876-0

CRISPR AAV vectors were generated by PackGene Biotech Co. (Guangzhou, China). For intramuscular injection into NSI mice, the animals were anesthetized by abdominal injection of 5% chloral hydrate, followed by
injection of CRISPR AAV vector (25 μL, 1.5E+12 vg) into the TA muscle transplanted with DMD–MDSCs.