CRISPR is a state-of-the-art genetic modification tool that can be used to knockout, mutate, or overexpress a gene of interest. CRISPR based research strategies have been widely employed across biological fields of study due to their powerful performance and relatively straightforward mechanism of action.
CRISPR gene editing requires the exogenous expression of the Cas9 enzyme as well as a 15-20nt guide RNA that is specifically targeted to the experiment’s gene of interest. Recent advances have made the design, construction, and use of the CRISPR systems increasingly convenient. PackGene’s pre-made Cas9 vectors build on this convenience by providing packaged AAV vectors for Cas9 expression at lighting fast turnaround times.
PackGene provides pre-made “off the shelf” CRISPR-AAV vectors that drive Cas9 expression under the EFS, miniCMV, or CMV broad-spectrum promoters as well as tissue-specific promoters in the AAV9 serotype. Our in stock pre-made products are shipped within one business day, making these products ideal for projects that are on a short timeline and require such standardized vectors.
PackGene provides high quality packaged AAVs for CRISPR Cas9 or CRISPR Cpf1 expression that can be directly applied in vivo and include a complete quality inspection report.
SKU |
Serotype |
Vector |
Titer & volume |
AAV9-A2 |
AAV9 |
EFS.SpCas9 |
≥1E+13 GC/mL, 0.1 mL |
AAV9-B2 |
EFS.SpCas9HF |
||
AAV9-B202 |
miniCMV.SpCas9HF |
||
AAV9-C202 |
miniCMV.SaCas9 |
||
AAV9-C2 |
CMV.SaCas9 |
||
AAV9-D2 |
CMV.SaCas9HF |
||
AAV9-G101 |
EFS.AsCpf1 |
||
AAV9-H101 |
EFS.LbCpf1 |
||
AAV9-G102 |
miniCMV.AsCpf1 |
||
AAV9-H102 |
miniCMV.LbCpf1 |
||
AAV9-C202* |
EFS.SaCas9 |
||
AAV9-C206 |
AAV9 |
Elastase I.SaCas9 |
≥1E+13 GC/mL, 0.1 mL |
AAV9-C207 |
rInsulin 2.SaCas9 |
||
AAV9-C208 |
mInsulin2.SaCas9 |
||
AAV9-C209 |
hDesmin.SaCas9 |
||
AAV9-C210 |
hSynapsin.SaCas9 |
||
AAV9-C212 |
MECP2.SaCas9 |
||
AAV9-D2* |
CMV.SaCas9HF |
||
AAV9-D202 |
EFS.SaCas9HF |
||
AAV9-D206 |
Elastase I.SaCas9HF |
||
AAV9-D207 |
rInsulin 2.SaCas9HF |
||
AAV9-D208 |
mInsulin2.SaCas9 |
||
AAV9-D209 |
hDesmin.SaCas9 | ||
AAV9-D210 | hSynapsin.SaCas9 | ||
AAV9-D212 | MECP2.SaCas9 |
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PackGene’s off the shelf Cas9 and Cpf1 vectors are packaged in AAV9, and can be directly, conveniently, and efficiently used for in vivo experiments. Each off the shelf product is delivered with a complete quality inspection report.
Injection Method: Intramuscular Injection
Recommended Dose: 25 μL, 1.5E+12 vg/ml
Targeting Site: muscle
Animal Model: DMD mouse model
Journal: Genome Medicine, 2021 (IF=15.266)
Paper Title: In vivo genome editing in mouse restores dystrophin expression in Duchenne muscular dystrophy patient muscle fibers
DOI: 10.1186/s13073-021-00876-0

CRISPR AAV vectors were generated by PackGene Biotech Co. (Guangzhou, China). For intramuscular injection into NSI mice, the animals were anesthetized by abdominal injection of 5% chloral hydrate, followed by
injection of CRISPR AAV vector (25 μL, 1.5E+12 vg) into the TA muscle transplanted with DMD–MDSCs.
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