π-Omega™ Plasmid DNA High-yield Production Platform
PackGene’s proprietary π-Omega™ Plasmid DNA High-yield Platform increases the plasmid yield by more than 3 times through plasmid backbone modifications. Production scale can reach up to 200L, and single-batch production yields can reach up to 100 grams. As a key raw material in AAV production, plasmid DNA cost reduction greatly reduces AAV production cost, while simultaneously meeting the growingly plasmids demand in the mRNA therapeutic field.
Advantages of the π-Omega™ Plasmid DNA High-yield Platform:
- Cost efficient: Greatly reduces cost by two-step chromatography
- Increase Yield: High-yield plasmid production driven by proprietary plasmid backbone modification
- Quality: Strain suitable for mRNA plasmid fermentation (maintaining the stability of polyA)
Intellectual property:
PackGene has one patents pending.
Increases Yield about 3-4 times by Molecular Modification on Plasmid
Two-step Purification Process to Improve Plasmid Quality
| Items | 3-step | 2-step |
| HCD | ＜5ug/mg | ＜2ug/mg |
| Supercoil | ＞95% | ＞95% |
| Endotoxin | ＜10EU/mg | ＜10EU/mg |
| HCP | N/A | N/A |
| Recovery | ＞40% | ＞50% |
| Remark | stability | High requirements for upstream stability |
Lower HCD & Higher Recovery Rate
Yield of plasmid maintains in the same range
