π-Omega™ Plasmid DNA High-yield Production Platform

PackGene’s proprietary π-Omega™ Plasmid DNA High-yield Platform has increased the plasmid yield by more than 3 times through the plasmid backbone modification. The production scale can reach to 200L, and the single-batch production yield can reach to 100 grams. As a key raw material in AAV production, plasmid DNA cost reduction has greatly reduced AAV production cost, while meeting the growingly plasmids demand in the mRNA therapeutic field.
Advantages:
- Greatly reduces cost by two-step chromatography
- High-yield plasmid production through the plasmid backbone modification
- Strain suitable for mRNA plasmid fermentation (maintaining the stability of polyA)
Interested in our AAV services? Please contact us for a free consultation or submit a request for a quotation.
One patents is pending.

Increases Yield about 3-4 times by Molecular Modification on Plasmid

Two-step Purification Process to Improve Plasmid Quality
Items | 3-step | 2-step |
HCD | <5ug/mg | <2ug/mg |
Supercoil | >95% | >95% |
Endotoxin | <10EU/mg | <10EU/mg |
HCP | N/A | N/A |
Recovery | >40% | >50% |
Remark | stability | High requirements for upstream stability |
Lower HCD & Higher Recovery Rate

Yield of plasmid maintains in the same range