PackGene’s proprietary π-Omega Plasmid DNA High-yield Platform increases plasmid yield by more than 3 times through plasmid backbone modifications. Production scale can reach up to 200L, and single-batch production yields can reach up to 100 grams. As a key raw material in AAV production, plasmid DNA cost reduction greatly reduces AAV production cost while simultaneously meeting the growingly plasmids demand in the mRNA based therapeutics.
![icon-1](https://static.packgene.com/wp-content/uploads/2023/09/icon-1.png)
Cost Efficient
Greatly reduces cost by two-step chromatography
![icon-4](https://static.packgene.com/wp-content/uploads/2023/09/icon-4.png)
Increase Yield
High-yield plasmid production driven by proprietary plasmid backbone modifications
![icon-3](https://static.packgene.com/wp-content/uploads/2023/09/icon-3.png)
Quality
Strain suitable for mRNA plasmid fermentation (maintaining the stability of polyA)
One patent is pending.
![Increases Yield about 3-4 times by Molecular Modification on Plasmid Increases Yield about 3-4 times by Molecular Modification on Plasmid](https://static.packgene.com/wp-content/uploads/2022/11/图片14.jpg)
Increases Yield about 3-4 times by Molecular Modification on Plasmid
![Two-step Purification Process to Improve Plasmid Quality Two-step Purification Process to Improve Plasmid Quality](https://static.packgene.com/wp-content/uploads/2022/11/图片15.png)
Two-step Purification Process to Improve Plasmid Quality
Items | 3-step | 2-step |
---|---|---|
HCD | <5ug/mg | <2ug/mg |
Supercoil | >95% | >95% |
Endotoxin | <10EU/mg | <10EU/mg |
HCP | N/A | N/A |
Recovery | >40% | >50% |
Remark | stability | High requirements for upstream stability |
Lower HCD & Higher Recovery Rate
![Yield of plasmid maintains in the same range Yield of plasmid maintains in the same range](https://static.packgene.com/wp-content/uploads/2022/11/图片16-1.jpg)
Yield of plasmid maintains in the same range