mRNA, short for messenger RNA, is a crucial player in the process of protein synthesis. In this article, we will explore what mRNA is and how it works to facilitate the creation of proteins, which are essential for the function and survival of all living things.

What is mRNA?

To understand what mRNA is, it is important to first have a basic understanding of genetics. DNA – deoxyribonucleic acid – is the genetic material that contains the instructions for all life processes. It is organized into units called genes, which contain the information needed to produce specific proteins. mRNA is a single-stranded molecule that is transcribed from a gene on a DNA molecule. It carries a copy of the genetic information from DNA to the protein-making machinery in cells. Essentially, it serves as a messenger between DNA and the cell’s protein-making machinery.

How does mRNA work?

The process of protein synthesis involves two main stages: transcription and translation.

Transcription is the process by which a gene is copied into mRNA. It occurs in the nucleus of eukaryotic cells, which are cells that contain a nucleus and other membrane-bound organelles. In prokaryotic cells, which lack a nucleus, transcription occurs in the cytoplasm. During transcription, an enzyme called RNA polymerase unwinds the DNA double helix and separates the two strands. It reads the DNA ‘template’ strand and synthesizes a complementary mRNA molecule by adding nucleotides one by one. The three-letter code on the DNA strand – made up of four different nucleotide bases, A, T, C, and G – determines the order of nucleotides added to the mRNA molecule. Once the mRNA molecule is complete, it is processed and modified in various ways before it can be used to make a protein. This includes adding a cap to the 5′ end of the mRNA and a tail to the 3′ end, as well as removing certain sections of the mRNA that are not needed for protein synthesis. The modified mRNA molecule is then transported out of the nucleus and into the cytoplasm, where it encounters the protein-making machinery known as ribosomes. This is where the second stage of protein synthesis, translation, occurs.

During translation, the ribosome reads the nucleotide sequence on the mRNA molecule and uses it as a template to synthesize a protein. The ribosome binds to the mRNA molecule at the start codon – a specific sequence of three nucleotides that signals the beginning of a protein sequence – and moves along the mRNA molecule, reading each three-letter ‘codon’ and matching it with a specific amino acid. Amino acids are the building blocks of proteins. There are 20 different amino acids that can be combined in various ways to create different proteins. Each amino acid is attached to a molecule called a transfer RNA (tRNA), which has a sequence of three nucleotides – called an anticodon – that is complementary to the mRNA codon. The ribosome brings the tRNA molecules with the matching amino acids to the mRNA codons, and the amino acids are joined together in a specific sequence to form a protein. Once the ribosome reaches the stop codon – a specific sequence of three nucleotides that signals the end of a protein sequence – the mRNA molecule is released and the newly synthesized protein is folded into its final shape and transported to where it is needed in the cell.

In summary, mRNA is a crucial component of the process of protein synthesis. It is synthesized from a gene on a DNA molecule, and it carries a copy of the genetic information to the protein-making machinery in cells. This allows for the creation of specific proteins that are essential for the function and survival of all living things. Understanding the role of mRNA in protein synthesis is essential for understanding many biological processes and has important implications for the development of new treatments for genetic diseases.

PackGene is a world-leading AAV vector CRO and CDMO company. We provides custom mRNA production with superior quality, industry-leading timelines, a wide range of deliverables, and the most stringent quality control that can help to streamline the vaccine and drug development process and accelerate the translation of scientific discoveries into practical therapies from early research and development to GMP mRNA manufacturing. Learn more about PackGene's mRNA Services.

If you want more information about our mRNA services, please Contact Us or Request a Quote