J Virol. 2021 Aug
Junxian Ou, Zhonghua Zhou, Ruixue Dai, Jing Zhang, Shan Zhao, Xiaowei Wu, Wendong Lan, Yi Ren, Lilian Cui, Qiaoshuai Lan, Lu Lu, Donald Seto, James Chodosh, Jianguo Wu, Gong Zhang
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SARS-CoV-2 Spike vector The full-length S gene of SARS-CoV-2 strain Wuhan-Hu-1 (NC_045512.2) was cloned into a SARS-CoV-2 spike vector (PackGene, Guangzhou, China) and confirmed by DNA sequencing. Request Quote

Research Field: Covid-related

Keywords: COVID-19, SARS-CoV-2, receptor-binding domain (RBD), viral infectivity, ACE2 receptor, variants, mutation


The current pandemic of COVID-19 is caused by a novel coronavirus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The SARS-CoV-2 spike protein receptor-binding domain (RBD) is the critical determinant of viral tropism and infectivity. To investigate whether naturally occurring RBD mutations during the early transmission phase have altered the receptor binding affinity and infectivity, we first analyzed in silico the binding dynamics between SARS-CoV-2 RBD mutants and the human angiotensin-converting enzyme 2 (ACE2) receptor. Among 32,123 genomes of SARS-CoV-2 isolates (December 2019 through March 2020), 302 nonsynonymous RBD mutants were identified and clustered into 96 mutant types. The six dominant mutations were analyzed applying molecular dynamics simulations (MDS). The mutant type V367F continuously circulating worldwide displayed higher binding affinity to human ACE2 due to the enhanced structural stabilization of the RBD beta-sheet scaffold. The MDS also indicated that it would be difficult for bat SARS-like CoV to infect humans. However, the pangolin CoV is potentially infectious to humans. The increased infectivity of V367 mutants was further validated by performing receptor-ligand binding enzyme-linked immunosorbent assay (ELISA), surface plasmon resonance, and pseudotyped virus assays. Phylogenetic analysis of the genomes of V367F mutants showed that during the early transmission phase, most V367F mutants clustered more closely with the SARS-CoV-2 prototype strain than the dual-mutation variants (V367F+D614G), which may derivate from recombination. The analysis of critical RBD mutations provides further insights into the evolutionary trajectory of early SARS-CoV-2 variants of zoonotic origin under negative selection pressure and supports the continuing surveillance of spike mutations to aid in the development of new COVID-19 drugs and vaccines.

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