Plasmid DNA High-yield Production Platform
PackGene’s proprietary π-Omega Plasmid DNA High-yield Platform increases plasmid yield by more than 3 times through plasmid backbone modifications. Production scale can reach up to 200L, and single-batch production yields can reach up to 100 grams. As a key raw material in AAV production, plasmid DNA cost reduction greatly reduces AAV production cost while simultaneously meeting the growingly plasmids demand in the mRNA based therapeutics.
Cost Efficient
Greatly reduces cost by two-step chromatography
Increase Yield
High-yield plasmid production driven by proprietary plasmid backbone modifications
Quality
Strain suitable for mRNA plasmid fermentation (maintaining the stability of polyA)
One patent is pending.
Increases Yield about 3-4 times by Molecular Modification on Plasmid
Two-step Purification Process to Improve Plasmid Quality
| Items | 3-step | 2-step |
|---|---|---|
| HCD | ＜5ug/mg | ＜2ug/mg |
| Supercoil | ＞95% | ＞95% |
| Endotoxin | ＜10EU/mg | ＜10EU/mg |
| HCP | N/A | N/A |
| Recovery | ＞40% | ＞50% |
| Remark | stability | High requirements for upstream stability |
Lower HCD & Higher Recovery Rate
Yield of plasmid maintains in the same range