How to Select AAVs
Step 1: Determine what specific AAV serotype you need.
There are more than 100 AAV serotypes and variants been discovered or created. We provide the mostly applied AAV serotypes for packaging. Each serotype presents its unique tissue-tropism profile. Until now, AAV9, AAVrh10 and newly emerging AAVPHP.B are ideal for systemically delivery or crossing Blood-Brain-Barrier into brain (basically mouse, AAVPHP.B is not yet examined in Non-Human Primate); AAV DJ is the best choice for in vitro experiment for its broad and high infectiousness in most cell lines. Check Table 1 and Table 2 below as reference for your experiment design (Red mark means highly recommended, but majorly depends on your experiment design), or you can contact us for technical support.
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Table1. AAV for in vivo experiment
Table 2. Relative in vitro infectivity *
Note: Infectivity rates normalized to AAV-2 = 100. ND = Not Determined
*Reference：Grimm, D. et al. (2008). J. Virol. 82: 5887-5911
*Reference：Grimm, D. et al. (2008). J. Virol. 82: 5887-5911
Step 2: Choose the vector fitting your experiments.
We have created a portfolio of Entry vectors need only one-step cloning for your experiments: CRISPR-Knock-out, CRISPR-Activation, Gene-Overexpression and shRNA Knock-Down system.
1. CRISPR-spCas9/saCas9/NmCas9/Cpf1
2. CRISPR-Activation system
3. Gene-Over expression system
4. shRNA Knock-Down system
5. miRNA-shRNA and other
2. CRISPR-Activation system
3. Gene-Over expression system
4. shRNA Knock-Down system
5. miRNA-shRNA and other
Table 3. Gene Over-expression