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How to Select AAVs

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Step 1: Determine what specific AAV serotype you need.
There are more than 200 AAV serotypes and variants that have been discovered or created, and this number is constantly increasing. Each of these serotypes holds several unique features including differences in cell and tissue tropisms, which is the ability of a serotype to infect specific tissues or cell type. Since the various AAV serotypes can differ substantially in tissue and cell type tropisms, selection of an appropriate serotype is an important step in the experiment or therapeutic design phase. For example, AAV DJ may often be the best choice for in vitro experiment due to its high infection rate across a broad range of cell lines, however, AAV9, AAVrh10 or AAVPHP.B may be better suited for in vivo experiments targeted at the central nervous system since these serotypes have been shown to penetrate the blood brain barrier.

At PackGene, we offer the most commonly used AAV serotypes for packaging. Please review Table 1 and Table 2 below as a quick references for during experiment or therapeutic design (the red mark means highly recommended, but majorly depends on your experiment design). In addition, our expert Technical Support team at PackGene would be happy to assist you in the selection of a serotype for your specific needs.

Liver Heart SK muscle Eye CNS CNS (BBB) Pancreas Lung T,B, Dendrital Cell

Table1. AAV for in vivo experiment

Step 2: Choose the vector fitting your experiments.
PackGene has developed a of entry vectors that require only one-step cloning for rapid customization. Available entry vectors have been designed for a multitude of experimental approached including:
  • CRISPR-Knock-out (CRISPR-spCas9/saCas9/NmCas9/Cpf1)
  • CRISPR-Activation
  • Gene over-expression
  • shRNA knock-down
  • miRNA-shRNA