Advanced Science. 2023 May 21
Xiaoyu Wang, Anke Zhang, Qian Yu, Zelin Wang, Junjie Wang, Penglei Xu, Yibo Liu, Jianan Lu, Jingwei Zheng, Huaming Li, Yangjian Qi, Jiahao Zhang, Yuanjian Fang, Shenbin Xu, Jingyi Zhou, Kaikai Wang, Sheng Chen, Jianmin Zhang
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AAV vector packaging For THBS1 or Control AAV delivery experiments, 2 μL of artificial CSF containing 10^13 genome copies per mL of AAV9-THBS1, or AAV-Con (AAV9, adeno-associated virus serotype 9; purchased from PackGene Biotech, Guangzhou), were injected into the cisterna magna CSF at a rate of 2 μL min−1. Request Quote

Research Field: CNS

AAV Serotype: AAV9

Dose: μL of artificial CSF containing 10^13 genome copies per mL

Routes of Administration: injected into the cisterna magna CSF

Targeted organ: brain

Animal or cell line strain: C57BL/6 male mice (6–8 weeks old)

Abstract

Subarachnoid hemorrhage (SAH) is a devastating subtype of stroke with high mortality and disability rate. Meningeal lymphatic vessels (mLVs) are a newly discovered intracranial fluid transport system and are proven to drain extravasated erythrocytes from cerebrospinal fluid into deep cervical lymph nodes after SAH. However, many studies have reported that the structure and function of mLVs are injured in several central nervous system diseases. Whether SAH can cause mLVs injury and the underlying mechanism remain unclear. Herein, single-cell RNA sequencing and spatial transcriptomics are applied, along with in vivo/vitro experiments, to investigate the alteration of the cellular, molecular, and spatial pattern of mLVs after SAH. First, it is demonstrated that SAH induces mLVs impairment. Then, through bioinformatic analysis of sequencing data, it is discovered that thrombospondin 1 (THBS1) and S100A6 are strongly associated with SAH outcome. Furthermore, the THBS1-CD47 ligand-receptor pair is found to function as a key role in meningeal lymphatic endothelial cell apoptosis via regulating STAT3/Bcl-2 signaling. The results illustrate a landscape of injured mLVs after SAH for the first time and provide a potential therapeutic strategy for SAH based on mLVs protection by disrupting THBS1 and CD47 interaction.

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