FAQ

Yes, we can co-encapsulate gene-editing mRNA and sgRNA in LNP. We have successfully co-encapsulated SpCas9 mRNA with sgRNA, achieving robust gene-editing efficiency in Huh7 cells. We recommend using the LP01 LNP formulation, and we will provide updates as we test other formulations.

We use linearized DNA plasmids as templates for mRNA production. The gene can either be designed by our team using PackGene’s proprietary vectors and tools or provided by you. Once the design is finalized, we manage the gene and mRNA manufacturing process and deliver the mRNA as part of the fee-for-service agreement.

Yes, we can supply cGMP mRNA through our partnership with Kudo Biotechnology. For more information, please visit our GMP mRNA service webpage. Please check our GMP mRNA webpage.

The amount of mRNA depends on the desired expression level and target cells or tissues. For mouse injections, we have tested 0.5 mg of FLuc or EGFP mRNA in LNP per kg of mouse body weight, yielding strong expression. For specific genes, it’s best to try a few different doses to optimize the amount.

Generally, we recommend using 0.5 µg to 2.5 µg of mRNA per well in 6-well plates (about 2 mL of growth medium containing 1.0–3.5 x 10^6 cells). For transfection in 24-well plates, we typically use 0.5 µg to 1 µg of mRNA per well.

Yes, we can perform quality testing on mRNA provided by customers. Please refer to the mRNA QC tests we offer here.

To measure poly(A) tail length, we enzymatically digest the mRNA sample with RNases or other hydrolyzing agents, breaking it down into smaller fragments and releasing the adenosine-rich poly(A) tails. These fragments are then quantified using LC-MS.

Yes, we can subclone your ORF sequences into our proprietary vector, which includes the T7 promoter, UTR, and a 110A tail, for mRNA production.