FAQ

Yes, we offer codon optimization services. Please contact our technical support team for further details.

For producing Cap1 mRNA using co-transcriptional capping, the sequence must include “AGG” following the T7 promoter.

Yes, you can, but we will first evaluate your sequence to ensure compatibility with our IVT system. Please send your sequence to our technical support team for assessment.

Yes, we provide a capping efficiency assay as part of our mRNA QC services, using enzyme digestion and LC-MS methods. For more information, please contact our technical support team.

We assess capping efficiency using LC-MS. Although not part of our standard release QC test, we typically achieve >97% capping efficiency based on internal testing. Detailed information about the capping efficiency measurement method is available here.

We provide Cap1 capping through co-transcriptional capping by default. Alternatively, enzymatic capping for Cap1 is available at an additional cost.

The 5′ cap of mRNA is essential for several key processes in gene expression and stability:

Translation Initiation: The 5′ cap, typically a modified guanosine nucleotide (m7G), is recognized by translation initiation factors, facilitating ribosome binding and the start of translation.

Protection from Degradation: The cap shields the mRNA from exonucleases, which degrade RNA from the ends, thereby enhancing the molecule’s stability and lifespan.

Nuclear Export: The cap aids in exporting mRNA from the nucleus to the cytoplasm, where translation occurs, by interacting with nuclear export factors.

Translation Efficiency: It promotes efficient translation by ensuring correct ribosome positioning at the start codon, boosting the accuracy of protein synthesis.

mRNA Processing Regulation: The cap also influences mRNA maturation, affecting processes like splicing and polyadenylation, and can impact alternative splicing patterns.

We offer substitution of uridine bases with N1-methyl-pseudouridine (N1-me-Ψ). If you require other modified bases, please contact our technical support team to discuss your options.