AAV Library Service

Key Benefits

Service Detail

• Random Peptide Library: Loop Insertion & Display

  Insert random peptide sequences (up to 20 amino acids) at specific surface loops of the AAV capsid with combinatorial mutations. This strategy creates high-diversity variants ideal for modifying receptor binding sites and altering tissue tropism.

  

• Saturation Scanning Library: Site-Specific Optimization

  Systematically substitute all 20 amino acids at specific residues to map functional hotspots. This method is essential for fine-tuning capsid stability, analyzing structure-activity relationships (SAR), and improving immune evasion.

  

• DNA Shuffling Library: Directed Evolution

  We ligate pre-synthesized gene fragments from multiple serotypes to create a chimeric library. This mimics natural evolution to combine the best properties of different parents into a single, high-performance capsid (~100 ready-to-use capsid genes available).

  

• Precision Library: Rational & Combinatorial Design

  You define the specific nucleotide components and positions; we generate the library using precise synthesis. This allows for multi-site mutations in a combinatorial way, ensuring high fidelity without frameshifts or stop codons.

  

• Barcoded Library: NGS-Ready Tracking

  Each variant is labeled with a unique 6-20 nt barcode. This enables rapid, cost-effective identification of high-performing variants via Next-Generation Sequencing (NGS) and precise cell lineage tracking.

  

Performance

• Library quality: Near 100% Coverage & Uniformity

  We validate every library using Next-Generation Sequencing (NGS) to ensure >99% coverage of the theoretical diversity. Our synthesis and cloning protocols are optimized to produce highly uniform libraries, preventing bias towards specific variants and ensuring every capsid in your design has an equal chance of being screened.

  

• Low mispackaging rates: High-Fidelity Genotype-Phenotype Correlation

  A critical challenge in AAV library screening is "cross-packaging," where a genome is packaged into a mismatched capsid. We quantify this rate by introducing capsid genes with early stop codons; their detection in the viral pool serves as a precise proxy for mismatching. Using our proprietary AAV Packaging protocol, we consistently achieve a mispackaging rate of <0.1%. This ensures that the DNA sequence you recover via NGS accurately corresponds to the capsid that successfully transduced the target tissue.

  

• Screening Result: Proven In Vivo Success

  Our libraries deliver real-world results. In a recent study, our platform utilized advanced AI algorithms to successfully identify novel muscle-targeting capsids in Cynomolgus monkeys. The validation data (shown below) demonstrates significantly enhanced transduction in skeletal muscle compared to standard serotypes like AAV9, confirming the power of our  AAV Library Service to solve complex tropism challenges.

  

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