Optogenetics-rAAV

Overview
Optogenetics is a technology that is largely used to manipulate neuronal activity. AAVs are used to drive the expression of light-activated ion channels in select neurons. Fiber optics are then directed toward the cells that express these light-activated ion channels, and light can be applied to rapidly manipulate the influx of charged ions and subsequently drive changes in neuronal activity. Most commonly, the ion channel Channelrhodopsin-2 (ChR2) is used to activate neurons while the ion channel Halorhodopsin (NpHR) is used to inhibit neurons. This technology provides a mechanism for selective manipulation of neurons at precise timescales during physiological or behavioral experiments and serves as a powerful tool for neuronal circuit mapping.

PackGene provides pre-made “off the shelf” AAV systems to drive expression of select light activated ion channels used for optogenetics. These AAVs can be used directly, conveniently, and efficiently for in vivo experiments and come standard with a complete quality inspection report.

Products

Promoter type

Catalog No.

Promoter and Gene

EF1a AAV-OT031 ssAAV.EF1a- double floxed- EYFP
AAV-OT032 ssAAV.EF1a- double floxed- hChR2(H134R)-EYFP
AAV-OT035 ssAAV.EF1a- eNpHR 3.0- EYFP
AAV-OT036 ssAAV.EF1a eArch 3.0- EYFP
AAV-OT037 ssAAV.EF1a- ChR2- mCherry
AAV-OT038 ssAAV-EF1a-FLEX-ArchT-GFP
AAV-OT039 ssAAV-Ef1a-DIO C1V1 (tt)-TS-mCherry
CaMKlla AAV-OT132 ssAAV.CaMKlla- double floxed- hChR2(H134R)- EYFP
AAV-OT134 ssAAV.CaMKlla- DIO eNpHR 3.0- EYFP
AAV-OT135 ssAAV.CaMKlla- eNpHR 3.0- EYFP
AAV-OT137 ssAAV.CaMKlla- ChR2- mCherry
GFAP AAV-OT171 ssAAV.GFAP-double floxed-EYFP
AAV-OT174 ssAAV.GFAP-DIO eNpHR 3.0-EYFP
AAV-OT175 ssAAV.GFAP-eNpHR 3.0-EYFP
AAV-OT176 ssAAV.GFAP-eArch 3.0-EYFP
AAV-OT178 ssAAV.GFAP-FLEX-ArchT-GFP
AAV-OT179 ssAAV.GFAP104-DIO C1V1 (tt)-TS-mCherry
AAV

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