Plasmid Preparation

DNA
  • Unlock the potential of plasmid DNA across multiple therapeutic approaches, from gene and cell therapies, viral vector production, and immunotherapeutics to mRNA/DNA vaccines and nucleic acid drugs. PackGene provides plasmid preparation services that are ideal for any phase of the drug development cycle including basic discovery research through commercialization at any scale.

lab
academic promotion

PackGene Referral Program

Refer a friend, get 20% off — and they save 20% too!

Promo Code: New2025

Key Benefits

  • Flexible Sizing

    We offer production sizes from 100µg to 2g for preclinical grades of plasmid and up to 100g for GMP plasmid production.
  • Precision

    Our extensive quality control ensures precise construction of plasmid DNA.
  • Speed

    Our process optimization results in industry-leading production times with rapid delivery.
  • Wide Compatibility

    We offer plasmid production grades that are ideal for all drug development stages, from discovery research to commercialization at any scale. Choose from two plasmid grades for discovery and preclinical work. For clinical stage work, please see GMP plasmid manufacturing
  • Tailored QC Tests

    Our range of quality control tests for endotoxin levels, supercoil content, residual contaminants, and sequence fidelity can be customized for your workflow.
  • Support for Various Projects

    Our plasmids help enable cell transfection, protein expression, AAV packaging, mRNA production, viral vector production, vaccine development, antibody production, and other preclinical processes.

Service Details

Research Grade Preclinical Grade
Application Molecular cloning, protein expression, transfection, viral packaging for research Large scale viral packaging, animal studies
Scale 100µg-100mg 10mg-2g
Timeline as fast as 3 days as fast as 2-3 weeks

 

QC standards

Research Grade Preclinical Grade
Appearance Clear, colorless, no visible particulates
A260:280 1.8 – 2.0
Homogeneity Predominantly supercoiled >90±10% supercoil
Restriction Analysis Conforming to reference pattern
Residual RNA Undetected by SYBRGold
Residual E. coli DNA Quantitative PCR ≤1%
Endotoxin ≤100 EU/mg or ≤ 10EU/mg, optional LAL test* ≤10 EU/mg
Bioburden Testing Optional* No growth on agarose plate after 48 hours
Sequencing Optional* GOI
Antibiotics Free Production No Optional
Residual Host Protein <0.1% by HCP ELISA*
Animal Free Production* TSE/BSE*
Mycoplasma Contamination Quantitative PCR, Negative *
Material Archiving Yes (plasmid only) Yes (plasmid + bacteria) upon request *
pH Potentiometry 7.5~8.0, USP <791> *
Kan ELISA, <0.5ng/g *
Sterility USP <71>*
Osmolality  USP <785> *
Advanced Endotoxin Removal <0.005 EU/µg Available with additional charge
Dedicated purification resin Upon request*
Buffer ddH2O, TE or customer defined
Dispensing Upon request
Dedicated filtration membrane Sterile filtration*

*Tests marked need additional charges and time.

Resource

Expand More

Contact Us

Download

Login

Don't have an account? Please register
Account*
Password*
Code*
Refresh
Forgot password?
Logging in indicates that you have read and accepted the Registration Agreement and User Agreement
Log in with other accounts

New User Registration

Already have an account?
First Name*
Middle Name
Last Name*
Organization*
Organization Type*
Country/State*
Email Address*
Code*
Set Password*
Confirm password*
Refferal Code*

Reset Password

Return to
Email*
Code*
New password*
Confirm password*

Google Account Binding

Organization*
Organization Type*
Country/State*