Cell Reports. 2021 Jan 26
Shuai Lu, Xi-xiu Xie,Lei Zhao, Bin Wang, Jie Zhu, Ting-rui Yang, Guang-wen Yang, Mei Ji, Cui-ping Lv, Jian Xue, Er-hei Dai, Xi-ming Fu, Dong-qun Liu, Lun Zhang, Sheng-jie Hou, Xiao-lin Yu, Yu-ling Wang, Hui-xia Gao, Xue-han Shi, Chang-wen Ke, Bi-xia Ke, Chun-guo Jiang, and Rui-tian Liu
Products used in the paper Details Operation
D614 SARS-CoV-2 pseudoviruses (Cat#LV-nCov1); G614 SARS-CoV-2 pseudoviruses (Cat#LV-nCov1); ACE2-239T cells (Cat#nCov-3) D614 SARS-CoV-2 pseudoviruses (Cat#LV-nCov1); G614 SARS-CoV-2 pseudoviruses (Cat#LV-nCov1); ACE2-239T cells (Cat#nCov-3) Request Quote

Research Field: Covid-related

Keywords: COVID-19, SARS-CoV-2, vaccine, immunodominant epitope, neutralizing epitope


Although vaccines against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) are under development, the antigen epitopes on the virus and their immunogenicity are poorly understood. Here, we simulate the 3D structures and predict the B cell epitopes on the spike (S), envelope (E), membrane (M), and nucleocapsid (N) proteins of SARS-CoV-2 using structure-based approaches and validate epitope immunogenicity by immunizing mice. Almost all 33 predicted epitopes effectively induce antibody production, six of these are immunodominant epitopes in individuals, and 23 are conserved within SARS-CoV-2, SARS-CoV, and bat coronavirus RaTG13. We find that the immunodominant epitopes of individuals with domestic (China) SARS-CoV-2 are different from those of individuals with imported (Europe) SARS-CoV-2, which may be caused by mutations on the S (G614D) and N proteins. Importantly, we find several epitopes on the S protein that elicit neutralizing antibodies against D614 and G614 SARS-CoV-2, which can contribute to vaccine design against coronaviruses.

Popular Services

AAV Packaging Service

AAV Analytical Service

Vector Design