Neural Regen Res. 2021 Apr 23
Xue-Yan Zhang, Jun Li, Cai-Juan Li, Ying-Qi Lin, Chun-Hui Huang, Xiao Zheng, Xi-Chen Song, Zhu-Chi Tu, Xiao-Jiang Li, and Sen Yan
Products used in the paper Details Operation
AAV vector packaging To investigate the effects of mutant HTT on cortical neurons at the cellular level, we used the adeno-associated virus (AAV) vector to express N-terminal HTT (1–230 amino acids) containing either 23Q or 109Q and a dsRED fluorescence protein (RFP). The AAV vectors were packaged and amplified by PackGene Biotech (Guangzhou, China). The vector contained the cytomegalovirus (CMV) promoter to express transgenes in cultured cortical neurons. Request Quote

Research Field: CNS

Keywords: Axion-MEA, electrical activities, human disease model, Huntington’s disease, HTT, monkey neuron, morphometric analysis, mouse neuron, neurodegenerative diseases, primary culture

Targeted organ: primary cortical neurons

Animal or cell line strain: Wild-type C57BL/6 mice, cynomolgus monkey


In vitro cultures of primary cortical neurons are widely used to investigate neuronal function. However, it has yet to be fully investigated whether there are significant differences in development and function between cultured rodent and primate cortical neurons, and whether these differences influence the utilization of cultured cortical neurons to model pathological conditions. Using in vitro culture techniques combined with immunofluorescence and electrophysiological methods, our study found that the development and maturation of primary cerebral cortical neurons from cynomolgus monkeys were slower than those from mice. We used a microelectrode array technique to compare the electrophysiological differences in cortical neurons, and found that primary cortical neurons from the mouse brain began to show electrical activity earlier than those from the cynomolgus monkey. Although cultured monkey cortical neurons developed slowly in vitro, they exhibited typical pathological features-revealed by immunofluorescent staining-when infected with adeno-associated viral vectors expressing mutant huntingtin (HTT), the Huntington’s disease protein. A quantitative analysis of the cultured monkey cortical neurons also confirmed that mutant HTT significantly reduced the length of neurites. Therefore, compared with the primary cortical neurons of mice, cultured monkey cortical neurons have longer developmental and survival times and greater sustained physiological activity, such as electrophysiological activity. Our findings also suggest that primary cynomolgus monkey neurons cultured in vitro can simulate a cell model of human neurodegenerative disease, and may be useful for investigating time-dependent neuronal death as well as treatment via neuronal regeneration. All mouse experiments and protocols were approved by the Animal Care and Use Committee of Jinan University of China (IACUC Approval No. 20200512-04) on May 12, 2020. All monkey experiments were approved by the IACUC protocol (IACUC Approval No. LDACU 20190820-01) on August 23, 2019 for animal management and use.

Popular Services

AAV Packaging Service

AAV Analytical Service

Vector Design