Nanomicro Lett. 2021 Apr 13
Yong Yang, Yusi Peng, Chenglong Lin, Li Long, Jingying Hu, Jun He, Hui Zeng, Zhengren Huang, Zhi-Yuan Li, Masaki Tanemura, Jianlin Shi, John R. Lombardi, and Xiaoying Luo
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viral strain that express SARS-CoV-2 nucleocapsid phosphoprotein Two kinds of viral strains that encode the spike protein and nucleocapsid protein of SARS-CoV-2, called the VS (SARSCoV-2 spike glycoprotein were expressed, 3.3 × 10^7 copies mL− 1) and VN (SARS-CoV-2 nucleocapsid phosphoprotein were expressed, CBV30002, 5.03 × 10^8 copies mL− 1) strain here, were obtained from COBIOER BIOSCIENCES CO., LTD. and PackGene Biotech, respectively. Request Quote

Research Field: Covid-related

Keywords: SERS, SARS-CoV-2, Human ACE2, “Virus-trap” nanostructure, Single-virus detection


The current COVID-19 pandemic urges the extremely sensitive and prompt detection of SARS-CoV-2 virus. Here, we present a Human Angiotensin-converting-enzyme 2 (ACE2)-functionalized gold “virus traps” nanostructure as an extremely sensitive SERS biosensor, to selectively capture and rapidly detect S-protein expressed coronavirus, such as the current SARS-CoV-2 in the contaminated water, down to the single-virus level. Such a SERS sensor features extraordinary 106-fold virus enrichment originating from high-affinity of ACE2 with S protein as well as “virus-traps” composed of oblique gold nanoneedles, and 109-fold enhancement of Raman signals originating from multi-component SERS effects. Furthermore, the identification standard of virus signals is established by machine-learning and identification techniques, resulting in an especially low detection limit of 80 copies mL−1 for the simulated contaminated water by SARS-CoV-2 virus with complex circumstance as short as 5 min, which is of great significance for achieving real-time monitoring and early warning of coronavirus. Moreover, here-developed method can be used to establish the identification standard for future unknown coronavirus, and immediately enable extremely sensitive and rapid detection of novel virus.

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