Biochemical Pharmacology. 2023 Apr
Meng-Bing Chen, Meng-Hua Li, Li-Yuan Wu, Rong Wang, Xi Long, Liang Zhang, Wei Sun, Wei-Wei Guo, Yong Pan, Yun-Shi Zhang, Chang Lin, Xi Shi, Shi-Ming Yang
Products used in the paper Details Operation
AAV vector packaging The target gene overexpression adeno-associated virus (AAV) vector AAV8 [ssAAV.CMV.EGFP-P2A-mIL1R2.WPRE.SV40pA] (PackGene, Guangzhou, China) (IL1R2-AAV) was constructed and transduced into the inner ear through the semicircular canal to achieve gene transduction in cochlear auditory cells (with GFP-AAV as control). Request Quote

Research Field: hearing

Keywords: Noise-induced hearing loss, Oridonin, Interleukin 1 receptor type 2, Inflammasome, Inflammation, Interleukin 1 receptor type 1

AAV Serotype: AAV8

Routes of Administration: semicircular canal

Targeted organ: inner ear

Animal or cell line strain: Male C57BL/6J mice, aged 5–6 weeks


NOD-like receptor protein 3 (NLRP3) inflammasomes trigger the inflammatory cascades and participate in various inflammatory diseases, including noise-induced hearing loss (NIHL) caused by oxidative stress. Recently, the anti-inflammatory traditional medicine oridonin (Ori) has been reported to provide hearing protection in mice after noise exposure by blocking the NLRP3-never in mitosis gene A-related kinase 7 (NEK7)-inflammasome complex assembly. Using RNA sequencing analysis, we further elucidated that interleukin 1 receptor type 2 (IL1R2) may be another crucial factor regulated by Ori to protect NIHL. We observed that IL1R2 expression was localized in spiral ganglion neurons, inner and outer hair cells, in Ori-treated mouse cochleae. Additionally, we confirmed that ectopic overexpression of IL1R2 in the inner ears of healthy mice using an adeno-associated virus delivery system significantly reduced noise-induced ribbon synapse lesions and hearing loss by blocking the “cytokine storm” in the inner ear. This study provides a novel theoretical foundation for guiding the clinical treatment of NIHL.

Popular Services

AAV Packaging Service

AAV Analytical Service

Vector Design