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AAV Genome Sequencing by Nanopore

  • We provide Nanopore AAV Genome Sequencing, offering a comprehensive, high-resolution view of the entire AAV genome. Nanopore sequencing provides long-read, real-time analysis of AAV genomes, enabling comprehensive characterization of genetic integrity. This advanced method detects mutations, insertions, deletions, inverted terminal repeat (ITR) integrity, and structural variations within the AAV genome, ensuring high-fidelity vector construction for gene therapy applications. Unlike short-read sequencing, Nanopore technology delivers full-length genome coverage, allowing for precise identification of heterogeneity, contaminants, and unexpected rearrangements that could impact safety and efficacy.

Nanopore testing
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Key Benefits

  • Full-Length Resolution

  • High Accuracy & Sensitivity

  • Rapid Turnaround

Service Details

Our AAV Genome Sequencing by Nanopore service provides unparalleled depth of characterization for your AAV vectors, supporting critical stages of gene therapy development from research to manufacturing quality control.

Feature Detail
Minimum Titer 5.00E+12 vg/mL
Minimum Sample Volume 500µL
Turnaround Time 5-10 Business Days

Performance

  • Our Nanopore sequencing service offers detailed analysis of the AAV genome by precisely detecting mutations, insertions, and deletions, including those within critical ITR regions. This comprehensive genome evaluation ensures the fidelity and stability of the AAV vector, providing essential quality assurance for its therapeutic use and regulatory submissions.

    Nanopore-sequencing-reads-depth-from-5-ITR-to-3-ITRNanopore sequencing reads depth from 5‘ ITR to 3’ ITR

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