Description
Cre-Lox recombination is a highly versatile site-specific recombinase technology employed to perform deletions, insertions, translocations, and inversions at precise locations within cell DNA. Its adaptability allows for targeted DNA modifications tailored to specific cell types or triggered by external stimuli, utilized in both eukaryotic and prokaryotic systems. Particularly in neuroscience, Cre-lox recombination has facilitated the study of complex brain structures and neural circuits underlying cognition and behaviors. Widely utilized as an experimental tool, the Cre-loxP system enables conditional gene manipulation, offering spatial and temporal control for investigating genes of interest in tissue or cell-specific contexts. in this mRNA, Cre and EGFP genes are connected with P2A, thus the two reading frames will be translated into two proteins folding independently due to ribosome skipping This mRNA possesses a Cap1 structure, which is highly efficient in capping. It is fully substituted with N1-methyl-pseudo Uridine to improve expression and decrease immunogenicity. Additionally, the mRNA features a 110A tail in its sequence, resembling a mature mRNA.
| Gene name | Cre-EGFP |
|---|---|
| Function | Gene Editing tool |
| ORF sequence | ATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGACCTACGGCGTGCAGTGCTTCAGCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTCAAGATCCGCCACAACATCGAGGACGGCAGCGTGCAGCTCGCCGACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCACTACCTGAGCACCCAGTCCGCCCTGAGCAAAGACCCCAACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCATGGACGAGCTGTACAAGGCTAGCGGAAGCGGAGCCACTAACTTCTCCCTGTTGAAACAAGCAGGGGATGTCGAAGAGAATCCCGGGCCACCCAAGAAGAAGAGGAAGGTGTCCAATCTCCTGACTGTTCACCAGAACCTCCCTGCGCTGCCAGTAGATGCCACTAGCGATGAGGTCAGGAAAAATCTCATGGATATGTTTAGGGATAGACAGGCGTTTTCTGAACACACCTGGAAAATGCTGCTTAGCGTGTGCCGATCCTGGGCAGCCTGGTGTAAGCTGAACAATCGCAAATGGTTCCCCGCCGAGCCGGAGGACGTGCGCGATTACCTGCTGTATCTCCAGGCAAGAGGGCTGGCTGTCAAGACTATCCAGCAGCACTTGGGCCAACTGAATATGCTGCATCGACGCAGCGGGCTCCCCCGGCCTAGCGATTCAAACGCAGTCTCCCTTGTTATGAGGAGAATTAGAAAGGAAAACGTAGATGCGGGTGAGAGGGCTAAGCAGGCTCTCGCTTTTGAGCGGACTGATTTCGACCAGGTCAGATCCCTGATGGAGAACAGCGATCGGTGCCAGGACATCAGGAACCTCGCATTTCTGGGAATTGCATATAACACACTTCTGCGCATAGCTGAGATCGCCCGGATCAGAGTGAAAGACATCAGTCGAACGGACGGCGGCCGGATGCTTATTCATATTGGACGCACAAAGACATTGGTCAGCACCGCTGGCGTTGAAAAGGCCTTGTCCCTGGGCGTAACGAAGCTGGTGGAAAGATGGATCTCAGTGTCCGGCGTGGCTGACGACCCTAATAATTACTTGTTCTGTCGAGTGAGAAAAAACGGAGTCGCCGCGCCCTCTGCCACCAGCCAATTGAGTACACGGGCCCTTGAAGGGATCTTTGAGGCAACCCACCGACTCATATACGGAGCCAAGGATGACAGTGGCCAGAGGTATCTCGCCTGGTCAGGTCATTCTGCTAGGGTGGGGGCCGCACGAGACATGGCGCGGGCAGGAGTCTCCATACCAGAGATTATGCAAGCTGGAGGTTGGACAAATGTGAACATCGTTATGAACTATATCCGCAATCTTGACTCTGAAACCGGGGCCATGGTGAGACTGCTCGAAGATGGTGACTACCCATACGATGTTCCAGATTACGCTTAA |
| Species | Zebrafish |
| Cellular localization of the protein | internal SV40 NLS |
| Reference/PubMed link | REFERENCE 1 (bases 1 to 3609) AUTHORS Sztal, T.E., Zhao, M., Williams, C., Oorschot, V., Parslow, A.C., Giousoh, A., Yuen, M., Hall, T.E., Costin, A., Ramm, G., Bird, P.I., Busch-Nentwich, E.M., Stemple, D.L., Currie, P.D., Cooper, S.T., Laing, N.G., Nowak, K.J. and Bryson-Richardson, R.J. TITLE Zebrafish models for nemaline myopathy reveal a spectrum of nemaline bodies contributing to reduced muscle function JOURNAL Acta Neuropathol. 130 (3), 389-406 (2015) PUBMED 25931053 REFERENCE 2 (bases 1 to 3609) AUTHORS Hall, T.E. and Currie, P.D. TITLE Cre-lox in zebrafish JOURNAL Unpublished REFERENCE 3 (bases 1 to 3609) AUTHORS Hall, T.E. and Currie, P.D. TITLE Direct Submission JOURNAL Submitted (21-OCT-2013) Institute for Molecular Bioscience, University of Queensland, 306 Carmody Road, St Lucia, QLD 4067, Australia |
| Concentration | 1mg/mL |
| Storage Buffer | Rnase Free H2O |
| Gene length (bp) | 1866 |
| Molecular Weight (kDa) | 837 |
| Storage Temperature | -80°C |
| Shipping Condition | Dry ice |
| Appearance | Clear and colorless |
| Purity | Fragment analysis by capillary electrophoresis |
| Base Composition | N1meψ |
| Capping | Cap1 |
| Tag | N-HA |
| Stock | On-demand |
