Description
NmCas9, also known as Neisseria meningitidis Cas9, is a variant of the CRISPR-associated protein Cas9 derived from the bacterium Neisseria meningitidis. Like other Cas9 variants, NmCas9 is an RNA-guided endonuclease enzyme that is used in genome editing applications. NmCas9 has gained attention for its relatively small size compared to other Cas9 orthologs, making it advantageous for certain applications where size constraints are a consideration. Despite its smaller size, NmCas9 retains the ability to target specific DNA sequences and induce double-strand breaks, enabling precise genetic modifications. This mRNA possesses a Cap1 structure, which is highly efficient in capping. It is fully substituted with N1-methyl-pseudo Uridine to improve expression and decrease immunogenicity. Additionally, the mRNA features a 110A tail in its sequence, resembling a mature mRNA.
| Stock | In stock |
|---|---|
| Gene name | NmCas9 |
| Function | Gene Editing tool |
| ORF sequence | ATGGTGCCTAAGAAGAAGAGAAAGGTGGCTGCCTTCAAACCTAATTCAATCAACTACATCCTCGGCCTCGATATCGGCATCGCATCCGTCGGCTGGGCGATGGTAGAAATTGACGAAGAAGAAAACCCCATCCGCCTGATTGATTTGGGCGTGCGCGTATTTGAGCGTGCCGAAGTACCGAAAACAGGCGACTCCCTTGCCATGGCAAGGCGTTTGGCGCGCAGTGTTCGCCGCCTGACCCGCCGTCGCGCCCACCGCCTGCTTCGGACCCGCCGCCTATTGAAACGCGAAGGCGTATTACAAGCCGCCAATTTTGACGAAAACGGCTTGATTAAATCCTTACCGAATACACCATGGCAACTTCGCGCAGCCGCATTAGACCGCAAACTGACGCCTTTAGAGTGGTCGGCAGTCTTGTTGCATTTAATCAAACATCGCGGCTATTTATCGCAACGGAAAAACGAGGGCGAAACTGCCGATAAGGAGCTTGGCGCTTTGCTTAAAGGCGTAGCCGGCAATGCCCATGCCTTACAGACAGGCGATTTCCGCACACCGGCCGAATTGGCTTTAAATAAATTTGAGAAAGAAAGCGGCCATATCCGCAATCAGCGCAGCGATTATTCGCATACGTTCAGCCGCAAAGATTTACAGGCGGAGCTGATTTTGCTGTTTGAAAAACAAAAAGAATTTGGCAATCCGCATGTTTCAGGCGGCCTTAAAGAAGGTATTGAAACCCTACTGATGACGCAACGCCCTGCCCTGTCCGGCGATGCCGTTCAAAAAATGTTGGGGCATTGCACCTTCGAACCGGCAGAGCCGAAAGCCGCTAAAAACACCTACACAGCCGAACGTTTCATCTGGCTGACCAAGCTGAACAACCTGCGTATTTTAGAGCAAGGCAGCGAGCGGCCATTGACCGATACCGAACGCGCCACGCTTATGGACGAGCCATACAGAAAATCCAAACTGACTTACGCACAAGCCCGTAAGCTGCTGGGTTTAGAAGATACCGCCTTTTTCAAAGGCTTGCGCTATGGTAAAGACAATGCCGAAGCCTCAACATTGATGGAAATGAAGGCCTACCATGCCATCAGCCGTGCACTGGAAAAAGAAGGATTGAAAGACAAAAAATCCCCATTAAACCTTTCTCCCGAATTACAAGACGAAATCGGCACGGCATTCTCCCTGTTCAAAACCGATGAAGACATTACAGGCCGTCTGAAAGACCGTATACAGCCCGAAATCTTAGAAGCGCTGTTGAAACACATCAGCTTCGATAAGTTCGTCCAAATTTCCTTGAAAGCATTGCGCCGAATTGTGCCTCTAATGGAACAAGGCAAACGTTACGATGAAGCCTGCGCCGAAATCTACGGAGACCATTACGGCAAGAAGAATACGGAAGAAAAGATTTATCTGCCGCCGATTCCCGCCGACGAAATCCGCAACCCCGTCGTCTTGCGCGCCTTATCTCAAGCACGTAAGGTCATTAACGGCGTGGTACGCCGTTACGGCTCCCCAGCTCGTATCCATATTGAAACTGCAAGGGAAGTAGGTAAATCGTTTAAAGACCGCAAAGAAATTGAGAAACGCCAAGAAGAAAACCGCAAAGACCGGGAAAAAGCCGCCGCCAAATTCCGAGAGTATTTCCCCAATTTTGTCGGAGAACCCAAATCCAAAGATATTCTGAAACTGCGCCTGTACGAGCAACAACACGGCAAATGCCTGTATTCGGGCAAAGAAATCAACTTAGGCCGTCTGAACGAAAAAGGCTATGTCGAAATCGACCATGCCCTGCCGTTCTCGCGCACATGGGACGACAGTTTCAACAATAAAGTACTGGTATTGGGCAGCGAAAACCAAAACAAAGGCAATCAAACCCCTTACGAATACTTCAACGGCAAAGACAACAGCCGCGAATGGCAGGAATTTAAAGCGCGTGTCGAAACCAGCCGTTTCCCGCGCAGTAAAAAACAACGGATTCTGCTGCAAAAATTCGATGAAGACGGCTTTAAAGAACGCAATCTGAACGACACGCGCTACGTCAACCGTTTCCTGTGTCAATTTGTTGCCGACCGTATGCGGCTGACAGGTAAAGGCAAGAAACGTGTCTTTGCATCCAACGGACAAATTACCAATCTGTTGCGCGGCTTTTGGGGATTGCGCAAAGTGCGTGCGGAAAACGACCGCCATCACGCCTTGGACGCCGTCGTCGTTGCCTGCTCGACCGTTGCCATGCAGCAGAAAATTACCCGTTTTGTACGCTATAAAGAGATGAACGCGTTTGACGGTAAAACCATAGACAAAGAAACAGGAGAAGTGCTGCATCAAAAAACACACTTCCCACAACCTTGGGAATTTTTCGCACAAGAAGTCATGATTCGCGTCTTCGGCAAACCGGACGGCAAACCCGAATTCGAAGAAGCCGATACCCTAGAAAAACTGCGCACGTTGCTTGCCGAAAAATTATCATCTCGCCCCGAAGCCGTACACGAATACGTTACGCCACTGTTTGTTTCACGCGCGCCCAATCGGAAGATGAGCGGGCAAGGGCATATGGAGACCGTCAAATCCGCCAAACGACTGGACGAAGGCGTCAGCGTGTTGCGCGTACCGCTGACACAGTTAAAACTGAAAGACTTGGAAAAAATGGTCAATCGGGAGCGCGAACCTAAGCTATACGAAGCACTGAAAGCACGGCTGGAAGCACATAAAGACGATCCTGCCAAAGCCTTTGCCGAGCCGTTTTACAAATACGATAAAGCAGGCAACCGCACCCAACAGGTAAAAGCCGTACGCGTAGAGCAAGTACAGAAAACCGGCGTATGGGTGCGCAACCATAACGGTATTGCCGACAACGCAACCATGGTGCGCGTAGATGTGTTTGAGAAAGGCGACAAGTATTATCTGGTACCGATTTACAGTTGGCAGGTAGCGAAAGGGATTTTGCCGGATAGGGCTGTTGTACAAGGAAAAGATGAAGAAGATTGGCAACTTATTGATGATAGTTTCAACTTTAAATTCTCATTACACCCTAATGATTTAGTCGAGGTTATAACAAAAAAAGCTAGAATGTTTGGTTACTTTGCCAGCTGCCATCGAGGCACAGGTAATATCAATATACGCATTCATGATCTTGATCATAAAATTGGCAAAAATGGAATACTGGAAGGTATCGGCGTCAAAACCGCCCTTTCATTCCAAAAATACCAAATTGACGAACTGGGCAAAGAAATCAGACCATGCCGTCTGAAAAAACGCCCGCCTGTCCGTTACCCATACGATGTTCCAGATTACGCTGCAGCTCCAGCAGCGAAGAAAAAGAAGCTGGATTAA |
| Species | Neisseria meningitidis |
| Cellular localization of the protein | N-terminal and C-terminal NLS |
| Reference/PubMed link | REFERENCE 1 |
| Concentration | 1mg/mL |
| Storage Buffer | Rnase Free H2O |
| Gene length (bp) | 3333 |
| Molecular Weight (kDa) | 1396 |
| Storage Temperature | -80°C |
| Shipping Condition | Dry ice |
| Appearance | Clear and colorless |
| Purity | Fragment analysis by capillary electrophoresis |
| Base Composition | N1meψ |
| Capping | Cap1 |
