Description
The ALC-0315 LNP formulation, containing enhanced green fluorescent protein mRNA, is developed using a standard LNP formulation with the key ionizable lipid ALC-0315. The cargo, EGFP (N1meψ) mRNA, has undergone full modification of all U bases with N1-methyl-pseudo-uridine. Upon translation, EGFP (N1meψ) mRNA generates enhanced green fluorescent protein, which emits fluorescence at 507nm upon excitation at 488nm. This mRNA sequence is widely employed as a fluorescence reporter in mRNA therapeutics and delivery system development endeavors. This formulation acts as a reliable control for evaluating the suitability of the ALC-0315 LNP formulation for your mRNA product in both in vitro and in vivo experiments.
| Gene name | EGFP (N1meψ) |
|---|---|
| LNP ionizable lipid | ALC-0315 |
| Concentration | 0.2 mg/mL |
| Storage Buffer | 5% Sucrose in PBS |
| ORF length (bp) | 720 |
| mRNA Molecular Weight (kDa) | 407 |
| Storage Temperature | -80°C |
| Shipping Condition | Dry ice |
| Handling | Avoid freeze and thaw |
| Appearance | Clear and colorless |
| Purity | Fragment analysis by capillary electrophoresis |
| Base Composition | N1meψ |
| Capping | Cap1 |
| Stock | In stock |
