Which AAV serotype should I use?

Oct 31, 2022

There are more than 10 serotypes of AAV that have been discovered or designed (AAV1/2/3/4/5/6/7/8/9/PHP.eB/PHP.S/rh10/DJ2-retro). Due to the difference in amino acid sequence and spatial structure of the capsid protein, the recognition and binding of cell surface receptors vary, resulting in different infection efficiency.

The infection efficiency of AAV serotypes of various tissues reported varies between research papers. For target tissues with fewer literature, it is recommended that researchers apply pre-experiment to determine the most ideal serotype.

Each serotype presents its unique tissue-tropism profile. Until now, AAV9, AAVrh10 and newly emerging AAVPHP.B are ideal for systemically delivery or crossing Blood-Brain-Barrier into the brain (basically mouse, AAVPHP.B is not yet examined in Non-Human Primates); AAV DJ is the best choice for in vitro experiment for its broad and high infectiousness in most cell lines. Check Table below as references for your experiment design .

 

Liver Heart SK muscle Eye CNS CNS (BBB) Pancreas Lung T,B, Dendrital Cell
AAV1 √ √ √
AAV2 √ √ √ √
AAV3 √ √ √ √
AAV4 √ √ √
AAV5 √ √ √ √
AAV6 √ √ √
AAV7 √ √
AAV8 √ √ √ √
AAV9 √ √ √ √ √ √ √ √
AAVrh10 √ √ √ √ √ √ √ √
AAVDJ √ √
AAVPHP.B √ √

PackGene provides superior quality AAV packaging services to support your AAV-based programs. We have developed a series of proprietary technologies that greatly improve AAV production outcomes including titer, purity, potency, and consistency.

We offer multiple serotypes to meet your research needs, and strive to achieve rapid turnaround and affordable prices while maintaining the highest quality. We are committed to delivering AAVs at guaranteed quantities and within the quoted project lead time. In a case where our quantity and efficiency guarantee is not met, we will refund 5% of the total order cost as an account credit that may be applied to your next order.

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