Viruses. 2022 Sep 11
Xuyang Wang, Yumeng Zhang, Yinyin Ben, Chao Qiu, Jing Wu, Wenhong Zhang, and Yanmin Wan
Products used in the paper Details Operation
AAV vector packaging A recombinant AAV8 vector carrying 1.3 copies of the HBV genome (genotype D, subtype ayw) was purchased from PackGene Biotech (Guangzhou, China). Request Quote

Research Field: liver

Keywords: anti-HBc IgG, AAV8-1.3HBV, HBsAg, HBV DNA, anti-HBc mAb

AAV Serotype: AAV8

Dose: To establish an AAV/HBV infected mouse model, the mice were infected with AAV8-1.3HBV (5 x10^10 GC/mouse, reconstituted in 200uL sterile PBS) via tail vein injection.

Routes of Administration: tail vein injection

Targeted organ: liver

Animal or cell line strain: Six- to eight-week-old C57BL/6N and C57BL/6J mice


Anti-HBc IgG is usually recognized as a diagnostic marker of hepatitis B, while the functional role anti-HBc IgG in HBV infection has not been fully elucidated. In this study, we firstly investigated the relationship between the anti-HBc IgG responses and the replication of HBV using AAV8-1.3HBV infected C57BL/6N mice. Our data showed that the anti-HBc IgG responses at the early phase of infection correlated negatively with the concentrations of circulating HBsAg and HBV DNA at both the early and chronic phases of infection. This observation was confirmed by an independent experiment using AAV8-1.3HBV infected C57BL/6J mice. Furthermore, to comprehend the potential causal relationship between the anti-HBc IgG responses and HBV infection, mice were treated with an anti-HBc monoclonal antibody at three days post AAV8-1.3HBV infection. Our data showed that the anti-HBc mAb significantly suppressed the fold increase of circulating HBsAg level, and the protective effect was not affected by NK cell depletion. Collectively, our study demonstrated that anti-HBc antibodies occurring at the early phase of HBV infection may contribute to the constraint of the virus replication, which might be developed as an immunotherapy for hepatitis B.

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