Research (Wash D C). 2022 Feb 4
Ting Cao, Changmin Shao, Xiaoyu Yu, Ruipei Xie, Chen Yang, Yulong Sun, Shaohua Yang, Wangjian He, Ye Xu, Qihui Fan, and Fangfu Ye
Products used in the paper Details Operation
SARS-CoV-2_del19AA-GFP pseudovirus Different concentrations of 2:22 × 10^6 TU/mL SARS-CoV-2_del19AA-GFP pseudovirus (PackGene, China) in ECM were added into the endothelial compartment when HUVEC were cultured for 12-24 h. Request Quote

Research Field: Covid-related

AAV Serotype: SARS-CoV-2_del19AA-GFP pseudovirus


SARS-CoV-2 has caused a severe pneumonia pandemic worldwide with high morbidity and mortality. How to develop a preclinical model for recapitulating SARS-CoV-2 pathogenesis is still urgent and essential for the control of the pandemic. Here, we have established a 3D biomimetic alveolus-on-a-chip with mechanical strain and extracellular matrix taken into consideration. We have validated that the alveolus-on-a-chip is capable of recapitulating key physiological characteristics of human alveolar units, which lays a fundamental basis for viral infection studies at the organ level. Using virus-analogous chemicals and pseudovirus, we have explored virus pathogenesis and blocking ability of antibodies during viral infection. This work provides a favorable platform for SARS-CoV-2-related researches and has a great potential for physiology and pathophysiology studies of the human lung at the organ level in vitro.

Popular Services

AAV Packaging Service

AAV Analytical Service

Vector Design