iScience. 2023 Sep 27
Yongyong Li, Jingshan Mo, Jing Liu, Ying Liang, Caiguanxi Deng, Zhangping Huang, Juan Jiang, Ming Liu, Xinmin Liu, Liru Shang, Xiafeng Wang, Xi Xie, Ji Wang
Products used in the paper Details Operation
Stable cell line: Human ACE2 overexpression stable HEK293T Human ACE2 overexpression stable HEK293T (hACE2-293T, PackGene Biotech) cells were cultured under an atmosphere of 5% CO2 at 37°C in Dulbecco’s modified Eagle’s medium (DMEM, #10-013-CVRC, Corining) supplemented with 10% inactivated FEB (#FSP500, ExCellBio), non-essential amino acids (NEAA, #11140-050, Gibco), 100 U/ml penicillin and 100 μg/ml streptomycin (#SV30010, HyClone). Request Quote

Research Field: DNA Vaccine

Keywords: Computational molecular modelling, Immune response, Immunology

Routes of Administration: intramuscular injection

Targeted organ: blood, liver

Animal or cell line strain: Mouse


“The constant emergence of mutated pathogens poses great challenges to the existing vaccine system. A screening system is needed to screen for antigen designs and vaccination strategies capable of inducing cross-protective immunity. Herein, we report a screening system based on DNA vaccines and a micro-electroporation/electrophoresis system (MEES), which greatly improved the efficacy of DNA vaccines, elevating humoral and cellular immune responses by over 400- and 35-fold respectively. Eighteen vaccination strategies were screened simultaneously by sequential immunization with vaccines derived from wildtype (WT) SARS-CoV-2, Delta, or Omicron BA.1 variant. Sequential vaccination of BA.1-WT-Delta vaccines with MEES induced potent neutralizing antibodies against all three viral strains and BA.5 variant, demonstrating that cross-protective immunity against future mutants can be successfully induced by existing strain-derived vaccines when a proper combination and order of sequential vaccination are used. Our screening system could be used for fast-seeking vaccination strategies for emerging pathogens in the future.”

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