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Mass Photometry: AAV Capsid Characterization Service

Precise, high-resolution assessment of full, partial, and empty capsid ratios

Mass Photometry

  • We offer advanced Mass Photometry (MP) testing for AAV capsid characterization. MP is a rapidly advancing biophysical technique that provides label-free, single-particle mass determination in solutions. This innovative method delivers rapid, cost-effective, high-resolution measurements to accurately assess AAV empty, partial, and full capsid ratios. Such insights are vital for ensuring the quality and consistency of your AAV products.

    MP offers significant advantages over traditional methods like Analytical Ultracentrifugation (AUC), Transmission Electron Microscopy (TEM), Dynamic Light Scattering (DLS), and droplet digital PCR (ddPCR). Its unique capabilities and impressive sensitivity, including the ability to provide in-process monitoring of AAV production and manufacturing, have allowed MP to quickly gain widespread adoption. It is rapidly becoming indispensable across AAV production and research, quality control, analytics, and AAV GMP manufacturing.

Mass Photometry
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Key Benefits

  • Full, Partial, Empty Capsid

  • In-process Sample Compatible

  • Minimal Sample Requirement

  • Rapid Analysis

  • Low Cost

  • GMP Compliance

Service Details

MP sample requirements and deliverables for AAV capsid characterization:

Feature Detail
Minimum Titer 1e11 vg/mL
Minimum Sample Volume 100 µL
Turnaround Time 2 Business Days
Data Report MP Graphic, Full/Partial/Empty Ratio

At PackGene, we use the state-of-the-art SamuxMP mass photometer from Refeyn, Ltd., for unparalleled AAV characterization. The SamuxMP is specifically designed for AAV analytics, precisely measuring light scattered by individual AAV particles as they land on a glass surface. This converts the signal into an accurate mass measurement for each particle.

This innovative, label-free method allows the SamuxMP to precisely distinguish and quantify full, partially filled, and empty AAV capsids, preserving their native state since they're analyzed directly in solution. It's also versatile, compatible with all AAV serotypes and common AAV buffers. Beyond crucial full/empty capsid ratio measurements, it also provides a rough capsid titer estimation.

PackGene Mass Photometry

Performance

  • Case study 1: In-process monitoring

    MP provides comprehensive monitoring of AAV capsid composition and determination of the full/empty ratio across the entire AAV purification/manufacturing process.

    In process monitoring

    MP Results

    Purification Empty Partial Full Total Particle Full%
    Step 1 126 18 49 193 25.40%
    Step 2 518 98 410 1026 40.00%
    Step 3 665 169 1815 2649 68.50%
    Step 4 192 81 410 683 60.00%
    Drug Substance 292 74 715 1081 66.10%
    Drug Product 253 92 634 979 64.80%

  • Case study 2: Comparison between AUC and MP

  • Comparison of AAV capsid analytical methods

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