Plasmid Preparation
Key Benefits
-
Flexible Sizing
We offer production sizes from 100µg to 2g for preclinical grades of plasmid and up to 100g for GMP plasmid production. -
Precision
Our extensive quality control ensures precise construction of plasmid DNA. -
Speed
Our process optimization results in industry-leading production times with rapid delivery. -
Wide Compatibility
We offer plasmid production grades that are ideal for all drug development stages, from discovery research to commercialization at any scale. Choose from two plasmid grades for discovery and preclinical work. For clinical stage work, please see GMP plasmid manufacturing -
Tailored QC Tests
Our range of quality control tests for endotoxin levels, supercoil content, residual contaminants, and sequence fidelity can be customized for your workflow. -
Support for Various Projects
Our plasmids help enable cell transfection, protein expression, AAV packaging, mRNA production, viral vector production, vaccine development, antibody production, and other preclinical processes.
Service Details
| Research Grade | Preclinical Grade | |
|---|---|---|
| Application | Molecular cloning, protein expression, transfection, viral packaging for research | Large scale viral packaging, animal studies |
| Scale | 100µg-100mg | 10mg-2g |
| Timeline | as fast as 3 days | as fast as 2-3 weeks |
QC standards
| Research Grade | Preclinical Grade | |
|---|---|---|
| Appearance | Clear, colorless, no visible particulates | |
| A260:280 | 1.8 – 2.0 | |
| Homogeneity | Predominantly supercoiled | >90±10% supercoil |
| Restriction Analysis | Conforming to reference pattern | |
| Residual RNA | – | Undetected by SYBRGold |
| Residual E. coli DNA | – | Quantitative PCR ≤1% |
| Endotoxin | ≤100 EU/mg or ≤ 10EU/mg, optional LAL test* | ≤10 EU/mg |
| Bioburden Testing | Optional* | No growth on agarose plate after 48 hours |
| Sequencing | Optional* | GOI |
| Antibiotics Free Production | No | Optional |
| Residual Host Protein | – | <0.1% by HCP ELISA* |
| Animal Free Production* | – | TSE/BSE* |
| Mycoplasma Contamination | – | Quantitative PCR, Negative * |
| Material Archiving | Yes (plasmid only) | Yes (plasmid + bacteria) upon request * |
| pH | – | Potentiometry 7.5~8.0, USP <791> * |
| Kan | – | ELISA, <0.5ng/g * |
| Sterility | – | USP <71>* |
| Osmolality | – | USP <785> * |
| Advanced Endotoxin Removal <0.005 EU/µg | – | Available with additional charge |
| Dedicated purification resin | – | Upon request* |
| Buffer | ddH2O, TE or customer defined | |
| Dispensing | Upon request | |
| Dedicated filtration membrane | – | Sterile filtration* |
*Tests marked need additional charges and time.
