
Brief intro:
- Author: Ting Cao, Changmin Shao, Xiaoyu Yu, Ruipei Xie, Chen Yang, Yulong Sun, Shaohua Yang, Wangjian He, Ye Xu, Qihui Fan, and Fangfu Ye
- Journal: Research (Wash D C)
- Doi: https://www.doi.org/10.34133/2022/9819154
- Publication Date: 2022 Feb 4
Products/Services used in the paper
Quotation shows PackGene:Different concentrations of 2:22 × 10^6 TU/mL SARS-CoV-2_del19AA-GFP pseudovirus (PackGene, China) in ECM were added into the endothelial compartment when HUVEC were cultured for 12-24 h.
Research Field:Covid-related
AAV Serotype:SARS-CoV-2_del19AA-GFP pseudovirus
Abstract
SARS-CoV-2 has caused a severe pneumonia pandemic worldwide with high morbidity and mortality. How to develop a preclinical model for recapitulating SARS-CoV-2 pathogenesis is still urgent and essential for the control of the pandemic. Here, we have established a 3D biomimetic alveolus-on-a-chip with mechanical strain and extracellular matrix taken into consideration. We have validated that the alveolus-on-a-chip is capable of recapitulating key physiological characteristics of human alveolar units, which lays a fundamental basis for viral infection studies at the organ level. Using virus-analogous chemicals and pseudovirus, we have explored virus pathogenesis and blocking ability of antibodies during viral infection. This work provides a favorable platform for SARS-CoV-2-related researches and has a great potential for physiology and pathophysiology studies of the human lung at the organ level in vitro.
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