Cap 1 N1meψ Sleeping Beauty SB-100 mRNA

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SKU: RNAP028 Category:

Description

The process of Sleeping Beauty transposition involves a cut-and-paste mechanism, wherein the transposable element “jumps” from one DNA molecule to another. Integration of the transposon into chromosomes serves as the foundation for sustained, potentially permanent, expression of transgenes in transgenic cells and organisms. The mRNA encoding the hyperactive variant of the Sleeping Beauty transposase is described by Mátés et al. (2009).

The SB-100 mRNA, as outlined in a study by Jin Z, et al. (2011) in Gene Therapy, is responsible for encoding the Sleeping Beauty transposase. This transposase, in conjunction with the Sleeping Beauty transposon, forms a DNA plasmid system utilized in therapeutic human cell genetic engineering. This mRNA possesses a Cap1 structure, which is highly efficient in capping. It is fully substituted with N1-methyl-pseudo Uridine to improve expression and decrease immunogenicity. Additionally, the mRNA features a 110A tail in its sequence, resembling a mature mRNA.

Cap 1 N1meψ Sleeping Beauty SB-100

Gene name

Sleeping Beauty SB-100

Function

Gene Editing tool

ORF sequence

ATGGGAAAATCAAAAGAAATCAGCCAAGACCTCAGAAAAAGAATTGTAGACCTCCACAAGTCTGGTTCATCCTTGGGAGCAATTTCCAAACGCCTGGCGGTACCACGTTCATCTGTACAAACAATAGTACGCAAGTATAAACACCATGGGACCACGCAGCCGTCATACCGCTCAGGAAGGAGACGCGTTCTGTCTCCTAGAGATGAACGTACTTTGGTGCGAAAAGTGCAAATCAATCCCAGAACAACAGCAAAGGACCTTGTGAAGATGCTGGAGGAAACAGGTACAAAAGTATCTATATCCACAGTAAAACGAGTCCTATATCGACATAACCTGAAAGGCCACTCAGCAAGGAAGAAGCCACTGCTCCAAAACCGACATAAGAAAGCCAGACTACGGTTTGCAACTGCACATGGGGACAAAGATCGTACTTTTTGGAGAAATGTCCTCTGGTCTGATGAAACAAAAATAGAACTGTTTGGCCATAATGACCATCGTTATGTTTGGAGGAAGAAGGGGGAGGCTTGCAAGCCGAAGAACACCATCCCAACCGTGAAGCACGGGGGTGGCAGCATCATGTTGTGGGGGTGCTTTGCTGCAGGAGGGACTGGTGCACTTCACAAAATAGATGGCATCATGGACGCGGTGCAGTATGTGGATATATTGAAGCAACATCTCAAGACATCAGTCAGGAAGTTAAAGCTTGGTCGCAAATGGGTCTTCCAACACGACAATGACCCCAAGCATACTTCCAAAGTTGTGGCAAAATGGCTTAAGGACAACAAAGTCAAGGTATTGGAGTGGCCATCACAAAGCCCTGACCTCAATCCTATAGAAAATTTGTGGGCAGAACTGAAAAAGCGTGTGCGAGCAAGGAGGCCTACAAACCTGACTCAGTTACACCAGCTCTGTCAGGAGGAATGGGCCAAAATTCACCCAAATTATTGTGGGAAGCTTGTGGAAGGCTACCCGAAACGTTTGACCCAAGTTAAACAATTTAAAGGCAATGCTACCAAATACTAG

Species

Synthetic

Cellular localization of the protein

Concentration

1mg/mL

Storage Buffer

Rnase Free H2O

Gene length (bp)

1023

Molecular Weight (kDa)

532

Storage Temperature

-80°C

Shipping Condition

Dry ice

Appearance

Clear and colorless

Purity

Fragment analysis by capillary electrophoresis

Base Composition

N1meψ

Capping

Cap1

Tag

N-HA

Stock

In stock

Additional information