Association of lncRNA AGAP11 with prognosis and malignant progression in triple-negative breast cancer
Brief intro:
- Author: Guihua Fu, Jingjing Song, Wei Wu, Xinyu Zhang, Dengyong Zhong
- Journal: Discover Oncology
- Doi: https://www.doi.org/10.1007/s12672-026-04727-5
- Publication Date: 2026/3/8
Abstract
Background
Triple-negative breast cancer (TNBC) is aggressive with poor prognosis and limited reliable prognostic markers.
Objectives
This study investigated the association between long non-coding RNA (lncRNA) ArfGAP With GTPase Domain, Ankyrin Repeat and PH Domain 11 (AGAP11) expression and clinicopathological characteristics and prognosis of early-stage TNBC patients, along with experimental validation of the regulatory effect of AGAP11 on TNBC cell malignancy and the tumor microenvironment through targeting miR-1269a.
Methods
This retrospective study examined 126 early-stage TNBC patients. AGAP11 and miR-1269a expression levels were detected using quantitative polymerase chain reaction (qPCR). The clinical significance of AGAP11 was analyzed. The impact of AGAP11 overexpression alone and in conjunction with miR-1269a overexpression, on the behavior of TNBC cells and the tumor microenvironment was examined.
Results
AGAP11 was down-regulated in TNBC tissues and cells (P < 0.001). Low AGAP11 expression was significantly associated with adverse clinicopathological characteristics (P < 0.05). Low AGAP11 was an independent risk factor for poor prognosis (HR = 0.254, P = 0.006) and predicted worse 5-year survival (P = 0.003). AGAP11 overexpression inhibited TNBC cell malignant activity (P < 0.05), and disrupted the homeostasis of the tumor microenvironment (P < 0.05). MiR-1269a was up-regulated in TNBC (P < 0.001). AGAP11 directly binds miR-1269a, and miR-1269a co-overexpression reversed inhibitory effects by AGAP11 (P < 0.05).
Conclusion
AGAP11 is a potential prognostic biomarker for TNBC and an important parameter for clinical stratification. Through the AGAP11/miR-1269a axis, it regulates TNBC cell behavior and the tumor microenvironment, which offers new insights for precision diagnosis and treatment.
About PackGene
PackGene Biotech is a world-leading CRO and CDMO, excelling in AAV vectors, mRNA, plasmid DNA, and lentiviral vector solutions. Our comprehensive offerings span from vector design and construction to AAV, lentivirus, and mRNA services. With a sharp focus on early-stage drug discovery, preclinical development, and cell and gene therapy trials, we deliver cost-effective, dependable, and scalable production solutions. Leveraging our groundbreaking π-alpha 293 AAV high-yield platform, we amplify AAV production by up to 10-fold, yielding up to 1e+17vg per batch to meet diverse commercial and clinical project needs. Moreover, our tailored mRNA and LNP products and services cater to every stage of drug and vaccine development, from research to GMP production, providing a seamless, end-to-end solution.
