AAV Genome Titer by ddPCR
Key Benefits
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Absolute Quantification
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Enhanced Precision
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Superior Sensitivity
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Robustness to Inhibitors
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Improved Reproducibility
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Multiplex Capability
Service Details
Our AAV Genome Titer by ddPCR service provides a robust and reliable solution for accurate viral load determination, crucial for the development and manufacturing of gene therapies.
| Feature | Detail |
|---|---|
| Minimum Titer | 1.00E+08 vg/mL |
| Minimum Sample Volume | 20µL |
| Primer and Probes | PackGene's standard or customer-provided |
| Turnaround Time | 5 Business Days |
AAV titer quantification by ddPCR typically starts with sample preparation: capsid lysis followed by DNase I digestion and heat treatment effectively releases the viral genomes. Next, during droplet generation, the PCR reaction mixture is precisely partitioned into approximately 20,000 nanoliter-sized, water-in-oil droplets using automated droplet generator. In the amplification phase, target sequences (such as AAV ITR regions) are PCR-amplified, with fluorescent probes specifically marking positive droplets containing AAV genomes. Finally, quantification is achieved by analyzing droplet data with Poisson statistics to determine the absolute concentration of genome copies per microliter.
Performance
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Our ddPCR service provides precise quantification of viral genome copies, ensuring accurate assessment of viral load. This is essential for determining effective dosages and maintaining batch-to-batch consistency in gene therapy applications.
