Return
Cap 1 N1meψ NmCas9 mRNA
NmCas9, also known as Neisseria meningitidis Cas9, is a variant of the CRISPR-associated protein Cas9 derived from the bacterium Neisseria meningitidis. Like other Cas9 variants, NmCas9 is an RNA-guided endonuclease enzyme that is used in genome editing applications. NmCas9 has gained attention for its relatively small size compared to other Cas9 orthologs, making it advantageous for certain applications where size constraints are a consideration. Despite its smaller size, NmCas9 retains the ability to target specific DNA sequences and induce double-strand breaks, enabling precise genetic modifications. This mRNA possesses a Cap1 structure, which is highly efficient in capping. It is fully substituted with N1-methyl-pseudo Uridine to improve expression and decrease immunogenicity. Additionally, the mRNA features a 110A tail in its sequence, resembling a mature mRNA.
SKU: RNAP010
title
content
Gene name
NmCas9
Function
Gene Editing tool
ORF sequence
ATGGTGCCTAAGAAGAAGAGAAAGGTGGCTGCCTTCAAACCTAATTCAATCAACTACATCCTCGGCCTCGATATCGGCATCGCATCCGTCGGCTGGGCGATGGTAGAAATTGACGAAGAAGAAAACCCCATCCGCCTGATTGATTTGGGCGTGCGCGTATTTGAGCGTGCCGAAGTACCGAAAACAGGCGACTCCCTTGCCATGGCAAGGCGTTTGGCGCGCAGTGTTCGCCGCCTGACCCGCCGTCGCGCCCACCGCCTGCTTCGGACCCGCCGCCTATTGAAACGCGAAGGCGTATTACAAGCCGCCAATTTTGACGAAAACGGCTTGATTAAATCCTTACCGAATACACCATGGCAACTTCGCGCAGCCGCATTAGACCGCAAACTGACGCCTTTAGAGTGGTCGGCAGTCTTGTTGCATTTAATCAAACATCGCGGCTATTTATCGCAACGGAAAAACGAGGGCGAAACTGCCGATAAGGAGCTTGGCGCTTTGCTTAAAGGCGTAGCCGGCAATGCCCATGCCTTACAGACAGGCGATTTCCGCACACCGGCCGAATTGGCTTTAAATAAATTTGAGAAAGAAAGCGGCCATATCCGCAATCAGCGCAGCGATTATTCGCATACGTTCAGCCGCAAAGATTTACAGGCGGAGCTGATTTTGCTGTTTGAAAAACAAAAAGAATTTGGCAATCCGCATGTTTCAGGCGGCCTTAAAGAAGGTATTGAAACCCTACTGATGACGCAACGCCCTGCCCTGTCCGGCGATGCCGTTCAAAAAATGTTGGGGCATTGCACCTTCGAACCGGCAGAGCCGAAAGCCGCTAAAAACACCTACACAGCCGAACGTTTCATCTGGCTGACCAAGCTGAACAACCTGCGTATTTTAGAGCAAGGCAGCGAGCGGCCATTGACCGATACCGAACGCGCCACGCTTATGGACGAGCCATACAGAAAATCCAAACTGACTTACGCACAAGCCCGTAAGCTGCTGGGTTTAGAAGATACCGCCTTTTTCAAAGGCTTGCGCTATGGTAAAGACAATGCCGAAGCCTCAACATTGATGGAAATGAAGGCCTACCATGCCATCAGCCGTGCACTGGAAAAAGAAGGATTGAAAGACAAAAAATCCCCATTAAACCTTTCTCCCGAATTACAAGACGAAATCGGCACGGCATTCTCCCTGTTCAAAACCGATGAAGACATTACAGGCCGTCTGAAAGACCGTATACAGCCCGAAATCTTAGAAGCGCTGTTGAAACACATCAGCTTCGATAAGTTCGTCCAAATTTCCTTGAAAGCATTGCGCCGAATTGTGCCTCTAATGGAACAAGGCAAACGTTACGATGAAGCCTGCGCCGAAATCTACGGAGACCATTACGGCAAGAAGAATACGGAAGAAAAGATTTATCTGCCGCCGATTCCCGCCGACGAAATCCGCAACCCCGTCGTCTTGCGCGCCTTATCTCAAGCACGTAAGGTCATTAACGGCGTGGTACGCCGTTACGGCTCCCCAGCTCGTATCCATATTGAAACTGCAAGGGAAGTAGGTAAATCGTTTAAAGACCGCAAAGAAATTGAGAAACGCCAAGAAGAAAACCGCAAAGACCGGGAAAAAGCCGCCGCCAAATTCCGAGAGTATTTCCCCAATTTTGTCGGAGAACCCAAATCCAAAGATATTCTGAAACTGCGCCTGTACGAGCAACAACACGGCAAATGCCTGTATTCGGGCAAAGAAATCAACTTAGGCCGTCTGAACGAAAAAGGCTATGTCGAAATCGACCATGCCCTGCCGTTCTCGCGCACATGGGACGACAGTTTCAACAATAAAGTACTGGTATTGGGCAGCGAAAACCAAAACAAAGGCAATCAAACCCCTTACGAATACTTCAACGGCAAAGACAACAGCCGCGAATGGCAGGAATTTAAAGCGCGTGTCGAAACCAGCCGTTTCCCGCGCAGTAAAAAACAACGGATTCTGCTGCAAAAATTCGATGAAGACGGCTTTAAAGAACGCAATCTGAACGACACGCGCTACGTCAACCGTTTCCTGTGTCAATTTGTTGCCGACCGTATGCGGCTGACAGGTAAAGGCAAGAAACGTGTCTTTGCATCCAACGGACAAATTACCAATCTGTTGCGCGGCTTTTGGGGATTGCGCAAAGTGCGTGCGGAAAACGACCGCCATCACGCCTTGGACGCCGTCGTCGTTGCCTGCTCGACCGTTGCCATGCAGCAGAAAATTACCCGTTTTGTACGCTATAAAGAGATGAACGCGTTTGACGGTAAAACCATAGACAAAGAAACAGGAGAAGTGCTGCATCAAAAAACACACTTCCCACAACCTTGGGAATTTTTCGCACAAGAAGTCATGATTCGCGTCTTCGGCAAACCGGACGGCAAACCCGAATTCGAAGAAGCCGATACCCTAGAAAAACTGCGCACGTTGCTTGCCGAAAAATTATCATCTCGCCCCGAAGCCGTACACGAATACGTTACGCCACTGTTTGTTTCACGCGCGCCCAATCGGAAGATGAGCGGGCAAGGGCATATGGAGACCGTCAAATCCGCCAAACGACTGGACGAAGGCGTCAGCGTGTTGCGCGTACCGCTGACACAGTTAAAACTGAAAGACTTGGAAAAAATGGTCAATCGGGAGCGCGAACCTAAGCTATACGAAGCACTGAAAGCACGGCTGGAAGCACATAAAGACGATCCTGCCAAAGCCTTTGCCGAGCCGTTTTACAAATACGATAAAGCAGGCAACCGCACCCAACAGGTAAAAGCCGTACGCGTAGAGCAAGTACAGAAAACCGGCGTATGGGTGCGCAACCATAACGGTATTGCCGACAACGCAACCATGGTGCGCGTAGATGTGTTTGAGAAAGGCGACAAGTATTATCTGGTACCGATTTACAGTTGGCAGGTAGCGAAAGGGATTTTGCCGGATAGGGCTGTTGTACAAGGAAAAGATGAAGAAGATTGGCAACTTATTGATGATAGTTTCAACTTTAAATTCTCATTACACCCTAATGATTTAGTCGAGGTTATAACAAAAAAAGCTAGAATGTTTGGTTACTTTGCCAGCTGCCATCGAGGCACAGGTAATATCAATATACGCATTCATGATCTTGATCATAAAATTGGCAAAAATGGAATACTGGAAGGTATCGGCGTCAAAACCGCCCTTTCATTCCAAAAATACCAAATTGACGAACTGGGCAAAGAAATCAGACCATGCCGTCTGAAAAAACGCCCGCCTGTCCGTTACCCATACGATGTTCCAGATTACGCTGCAGCTCCAGCAGCGAAGAAAAAGAAGCTGGATTAA
Species
Neisseria meningitidis
Cellular localization of the protein
N-terminal and C-terminal NLS
Reference/PubMed link
REFERENCE 1 CONSRTM Pathogen Informatics TITLE Direct Submission JOURNAL Submitted (18-DEC-2018) WTSI, Pathogen Informatics, Wellcome Trust Sanger Institute, CB10 1SA, United Kingdom
Concentration
1mg/mL
Storage Buffer
Rnase Free H2O
Gene length (bp)
3333
Molecular Weight (kDa)
1232.3
Storage Temperature
-80°C
Shipping Condition
Dry ice
Appearance
Clear and colorless
Purity
Fragment analysis by capillary electrophoresis
Base Composition
N1meψ
Capping
Cap1
Tag
N-HA
Stock
In stock