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HEK-293T/hACE2 Cell Line

The SARS-Cov-2 spike (Spike) glycoprotein infects the host by binding to the ACE2 receptor on the host cell surface. Using a 293T cell line overexpressing hACE2 with a novel coronavirus S protein to reconstitute a pseudoviral system that mimics the process of infection and entry of a new coronavirus into the human body is a very effective and practical strategy. Moreover, researchers can conduct this experiment in P2 labs (BSL-2 level), which to some extent relieves the resource constraint of P3 labs (BSL-3) around the world and makes it possible to conduct virus research experiments rapidly. Paijin Biologics provides researchers with a 293T cell line overexpressing hACE2 with a novel coronavirus S protein recombinant pseudovirus.

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The SARS-Cov-2 spike glycoprotein infects hosts by binding to ACE2 receptors on the host cell surface and is a key target for the development of diagnostic antibodies, therapeutic antibodies, vaccines and diagnostics. S-protein recombinant pseudoviruses are a class of recombinant retroviruses that can integrate other viral capsid glycoproteins to form exogenous viral capsids, but still maintain their own genomic identity.

S-protein recombinant pseudoviruses share the same infection mechanism as novel coronaviruses but are not pathogenic and can therefore be safely and reliably expressed and produced on a large scale. This allows researchers to establish a cellular assay system and high-throughput antibody screening platform using novel coronavirus S-protein recombinant pseudoviruses and 293T/hACE2 cells without relying on the BSL-3 laboratory.

The 293T/hACE2 cell line has undergone stringent quality control, including cell viability, sterility testing, mycoplasma testing, and ACE2 expression activity.

AAV-hACE2 animal modeling

Traditional animal modeling

Production Cycle

Short cycle within 14 days
Over 6 months

Process

Simple
Complicated steps

Histotropism

Specific distribution
Wide distribution

Characteristics of 293T/hACE2 cell line

  • Stable and reliable.
  • Mechanism of infection is the same as that of neo-coronavirus, but not pathogenic.
  • Capable of scale-up production and screening on a BSL-2 laboratory basis.
  • Sterility, mycoplasma and ACE2 activity assays.

Cell processing

  • Resuscitated cells: Thaw frozen tubes containing 2 mL of cell suspension by rapid shaking in a 37°C water bath and add 5 mL of culture medium and mix well. Centrifuge at 1000rpm for 5 minutes, discard the supernatant, add 4-6mL of complete medium and blow well. All cell suspensions were then added to a 10 cm dish and incubated overnight. The next day, change the solution and check the cell density.
  • Cell passaging: If the cell density reaches 80%-90%, passaging culture can be performed.

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