With optimized transfection conditions, a typical titer from one 15cm dish is 1E+11GC/ml to 1E+12 GC/ml, with each cell producing 2×10^4 to 1×10^5 viral particles. There are many variables that can influence this value, such as the size of the insert, the packaging cells, and the transfection efficiency.
The guaranteed titer of PackGene is 1E+13GC/ml as default, while we may also concentrate the titer if you need higher titer, but the highest titer is suggested no higher than 5E+13GC/ml for normal-yield AAV prep due to the proneness to aggregation. For low-yield AAV preps, the highest titer is suggested no higher than 1E+13GC/ml.
PackGene provides superior quality AAV packaging services to support your AAV-based programs. We have developed a series of proprietary technologies that greatly improve AAV production outcomes including titer, purity, potency, and consistency.
We offer multiple serotypes to meet your research needs, and strive to achieve rapid turnaround and affordable prices while maintaining the highest quality. We are committed to delivering AAVs at guaranteed quantities and within the quoted project lead time. In a case where our quantity and efficiency guarantee is not met, we will refund 5% of the total order cost as an account credit that may be applied to your next order.